USE OF PROTEIN ELECTROPHORESIS TO QUANTIFY RESISTANCE OF FLAX GENOTYPES TO POWDERY MILDEW DISEASE | ||
| Journal of Plant Production | ||
| Article 10, Volume 31, Issue 1, January 2006, Pages 213-224 PDF (2.98 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jpp.2006.235699 | ||
| Authors | ||
| A. A. Aly,; M. T.M. Mansour; E. M. Hussein | ||
| Plant Pathology Research Institute Agric. Res. Center, Giza, Egypt. | ||
| Abstract | ||
| Ten flax genotypes were evaluated for powdery mildew resistance under field conditions in 200212003 and L003/2004 growing seasons. Lines 42014, 421/43, and 421/60 showed the lowest ratings of disease severity, while line 110/3 showed the highest rating. The remaining genotypes showed intermediate ratings ranging from 40.93 to 57.89%. Proteins of cultivar seeds were separated by SOS-PAGE, and the obtained banding patterns were visualized by using the silver nitrate staining system. Data for powdery mildew ratings and amounts of protein fractions were entered into a computerized stepwise multiple regression. Using the predictors supplied by stepwise regression, a four-factor model was constructed to predict powdery mildew severity. This model showed that powdery mildew severity differences were due largely to the protein fractions nos. 33, 31, 6, and 53, which accounted for 95.43% of the total variation in severity ratings. This result indicates that SOS-PAGE of seed proteins may provide a supplementary assay to field tests to distinguish quantitatively between powdery mildew resistant or susceptible genotypes. | ||
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