MICROPROPAGATION AND SUITABLE PROTOCOL TO OVERCOME HYPERHYDRICITY OF Justicr'a brandegeana | ||
| Journal of Plant Production | ||
| Article 24, Volume 31, Issue 8, August 2006, Pages 5267-5280 PDF (184.18 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jpp.2006.236127 | ||
| Author | ||
| H. M. S. Aboshama, | ||
| Minufiya University. Genetic Engineering and Biotechnology Research Institute. Plant Biotechnology Department, Egypt. | ||
| Abstract | ||
| For micrOpropagation of Justicia brandegeana. shoot tips were excised from mature plants grown in the greenhouse. These explants were cultured on MS medium containing combination of BA and NAA. Hyperhydricity was a serious problem during the micropropagation of Justicr‘a brandegeana. The effects of different factors for over— coming of hyperhydricity were studied. This phenomenon was influenced by level and type of cytokinin. salts media. vessel aeration and agar concentrations. High BA concentrations (2.0 and 3.0 mgiL) gave multiple shoots but led to high incidence of hyperhydricity. WPM was significantly effective in controlling hyperhydricity in shrimp < /p> plant compared with MS medium. The treatment of 8.0giL agar with opening jars (weekly) gave the lowest hyperhydricity percentage (8.39%) without affecting shoot multiplication (11.20 shootiexplant). The developed shoots were transferred to half— MS medium for rooting. Two types of auxin were tested; IBA or NAA each at 0.0. 0.5 and 1.0 mgiL with or without 3.0 gll. AC. The treatment of 1.0 mgiL NM and 3.0gr'L AC produced the largest number of roots (4.60). The plantlets were transferred from the rooting medium and cultured in 1:1 peatmoss: sand mixture. The survival percentage was 73% after 5 weeks from transplanting. | ||
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