Abd El-Zaher, E., Tousson, E., Mostafa, A., M. El-Gaar, E. (2022). Production of endo Polysaccharides from cultivated Pleurotus eryngii fruiting bodies. test, 44(1), 135-144. doi: 10.21608/djs.2022.127995.1020
Eman Hasan Abd El-Zaher; Ehab M. Tousson; Azza A. Mostafa; Enas M. El-Gaar. "Production of endo Polysaccharides from cultivated Pleurotus eryngii fruiting bodies". test, 44, 1, 2022, 135-144. doi: 10.21608/djs.2022.127995.1020
Abd El-Zaher, E., Tousson, E., Mostafa, A., M. El-Gaar, E. (2022). 'Production of endo Polysaccharides from cultivated Pleurotus eryngii fruiting bodies', test, 44(1), pp. 135-144. doi: 10.21608/djs.2022.127995.1020
Abd El-Zaher, E., Tousson, E., Mostafa, A., M. El-Gaar, E. Production of endo Polysaccharides from cultivated Pleurotus eryngii fruiting bodies. test, 2022; 44(1): 135-144. doi: 10.21608/djs.2022.127995.1020

Production of endo Polysaccharides from cultivated Pleurotus eryngii fruiting bodies

Delta Journal of Science
Article 10, Volume 44, Issue 1, July 2022, Pages 135-144    PDF (748.94 K)
Document Type: Research and Reference
DOI: 10.21608/djs.2022.127995.1020
Authors
Eman Hasan Abd El-Zaher1; Ehab M. Tousson2; Azza A. Mostafa3; Enas M. El-Gaar* 4
1Microbiology Unit, Botany Department, Faculty of Science, Tanta University,Tanta, Egypt
2Cell Biology and Histology, Zoology Department, Faculty of Science, Tanta University, Tanta, Egypt
3Biological and Environmental Department, Faculty of Home Economics, Al Azhar University, Tanta, Egypt
4Microbiology Unit, Botany Department, Faculty of Science, Tanta University, Tanta, Egypt
Abstract
Pleurotus eryngii was cultivated on four different sterilized agro wastes as wheat, rice, sugarcane and wood. Wheat substrate gave the maximum yield of P. eryngii fruiting bodies (63.4 and 11.3 gm ) for 100 gram of substrate for first flush and second flush respectively. Also, cultivation of tested mushroom on wheat substrate produced high quantity of polysaccharides (0.703 mg/ml) which was colormetrically evaluated by Phenol-Sulfuric acid method. The structure of endopolysaccharides was studied by Fourier Transform-Infrared spereectroscopy (FT-IR) noticed presence of O-H, C-H, C-O-C, CH2 and C-O bonds that indicated the appearance of c1-c5 by 1H & 13C NMR. Polysacchraides analysis with HPLC indicated that it is consisting of glucose, galactose, glucournic acid, ribose, rhamnose and mannose. Polysaccharides were obtained frome Pleurotus eryngii approved high antioxidant activity by Di phenyl Pichryl hydrocarbon (DPPH) radical scavenging activity which increased by increasing the concentration of polysaccharides. Polysaccharides had IC50 at 0.32 mg/ml.
Keywords
Polysaccharides; Pleurotus eryngii; Di phenyl Pichryl hydrocarbon radical scavenging activity; Fourier Transform-Infrared spectroscopy; Nuclear Magnetic Resonance and Half Inhibation Concentration
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