THE GENOTOXIC AND CYTOTOXIC EFFECTS OF ABAMECTIN ON GERM CELLS AND BONE MARROW OF MALE MICE | ||
| Egyptian Journal of Zoology | ||
| Article 6, Volume 59, Issue 59, June 2013, Pages 81-100 | ||
| Document Type: Original Research Papers | ||
| DOI: 10.12816/0001306 | ||
| Author | ||
| Iman Abd El Moneim Darwish* | ||
| Zoology Department, Women`s College for Arts, Science and Education, Ain Shams University, Cairo, Egypt | ||
| Abstract | ||
| Abamectin is a broad-spectrum anti-parasitic agent in veterinary and human medicine. The objective of this study was the evaluation of potential mutagenicity of abamectin through meiotic chromosomal division and the apoptosis in bone marrow of mice. Animals received single oral treatment of 0.8 mg/Kg vody weight abamectin. Meiotic chromosomes were prepared after 6 hours, 2, 5, 10, 12 and 21 days to cover the different phases of meiotic division. The bone marrow samples were taken from mice and the apoptotic cells were stained with propodium iodide and examined with fluorescence microscope. The frequency of cells with structural chromosome aberrations in testes peaked at 12 days after treatment. This indicates that the preleptotene when DNA synthesis occurred was the most sensitive stage of meiotic prophase. The types of aberrations recorded were structural, including X-Y and autosomal univalent, chromosomal rearrangements in the form of quadrivalent chain (CIV), hexavalent (CVI), Robertsonian translocations, centromeric attenuation, ring, acentric fragments, end to end association and numerical in the form of diploid, triploid, tetraploid and aneuploid. The percentage of apoptotic cells increased after two days, then begin to decrease with increasing the treatment time and the percentages reached 16.33%, 40.16%, 38.66%, 34.66%, 14.83% and 13.33% after 6 hours, 2, 5, 10, 12 and 21 days, respectively. In conclusion, the increase in the chromosomal aberrations in testis and the apoptotic cells in bone marrow revealed high cytotoxic and genotoxic potential of abamectin on mice. | ||
| Keywords | ||
| Abamectin; Meiotic division; chromosomal aberrations; Apoptotic cells | ||
|
Statistics Article View: 174 |
||
