INTENSIVE PRODUCTION OF DIHAPLOID PLANTS FROM Cucurbita pepo L. THROUGH ANTHER CULTURE TECHNIQUE. | ||
| Journal of Plant Production | ||
| Article 5, Volume 30, Issue 12, December 2005, Pages 7885-7893 PDF (140.56 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jpp.2005.237932 | ||
| Authors | ||
| M. A. Badawi,1; E. I. Metwally2; S. S. Taha1; M. O. Arafeh1 | ||
| 1• Department of Vegetable Crops, Faculty of Agric., Cairo University, Egypt. | ||
| 2Horticulture Department, Faculty of Agriculture, Tanta University, Kafr EI-Sheikh, Egypt | ||
| Abstract | ||
| Production of haploid plants using techniques such as anthers culture has long been considered important fOf gene~ic studies and plant breeders. Since haploid plants carry only one allele of each gene, recessive characteristics are apparent. These haploid plants can be used to produce homozygous dihaploid plants useful for plant breeding. This studv is concerned with the production of dihaploids plants of Cucurbita p < /em>epo L. Anthers at mid to late uninucleate microspore stage without filament were excised from sterilized buds of three genotypes of summer squash: i. e Gabbla, Eskandarani and Rosina F,. Anthers were cultured on two different induction media. It was found that both media and cultivars affected callus induction and plantlets regeneration. The cullivar Gabbla gave the maximum callus weight (0.450 g) on MS medium supplemented with 5.0 mg II 2.4-0 and 90 gIl sucrose. On the other hand, the cultivar Eskandarani gave the maximum number of plantlets per dish (21.3)and per callus (4.3). Plantlets were acclimatized in the controlled environment. In each genotype, the root tips of plantlets were examined for ploidy level. Results reveal that there was 50% haploid plants in Eskandarani and Gabbla cultivars while Rosina F 1 had 40% haploid plants. | ||
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