Mohamed, S., Ross, S., Mohamed, N. (2022). EXPLORATION OF COMPONENTS CONTRIBUTING TO POTENT CYTOTOXICITY OF GARDENIA THUNBERGIA L. F. AGAINST HUMAN LEUKEMIA AND HEPATOMA. test, 45(1), 153-162. doi: 10.21608/bfsa.2022.239374
Shaymaa M. Mohamed; Samir Ross; Nesma M. Mohamed. "EXPLORATION OF COMPONENTS CONTRIBUTING TO POTENT CYTOTOXICITY OF GARDENIA THUNBERGIA L. F. AGAINST HUMAN LEUKEMIA AND HEPATOMA". test, 45, 1, 2022, 153-162. doi: 10.21608/bfsa.2022.239374
Mohamed, S., Ross, S., Mohamed, N. (2022). 'EXPLORATION OF COMPONENTS CONTRIBUTING TO POTENT CYTOTOXICITY OF GARDENIA THUNBERGIA L. F. AGAINST HUMAN LEUKEMIA AND HEPATOMA', test, 45(1), pp. 153-162. doi: 10.21608/bfsa.2022.239374
Mohamed, S., Ross, S., Mohamed, N. EXPLORATION OF COMPONENTS CONTRIBUTING TO POTENT CYTOTOXICITY OF GARDENIA THUNBERGIA L. F. AGAINST HUMAN LEUKEMIA AND HEPATOMA. test, 2022; 45(1): 153-162. doi: 10.21608/bfsa.2022.239374

EXPLORATION OF COMPONENTS CONTRIBUTING TO POTENT CYTOTOXICITY OF GARDENIA THUNBERGIA L. F. AGAINST HUMAN LEUKEMIA AND HEPATOMA

Bulletin of Pharmaceutical Sciences Assiut University
Article 13, Volume 45, Issue 1, June 2022, Pages 153-162    PDF (902.96 K)
Document Type: Original Article
DOI: 10.21608/bfsa.2022.239374
Authors
Shaymaa M. Mohamed1; Samir Ross2, 3; Nesma M. Mohamed* 4
1Department of Pharmacognosy, Faculty of Pharmacy, Assiut University, Assiut 71515, Egypt
2National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, School of Pharmacy, The University of Mississippi, University, Mississippi 38677, USA
3Department of BioMolecular Sciences, Division of Pharmacognosy, School of Pharmacy, University of Mississippi, University, Mississippi 38677, USA
4Department of Pharmacognosy, Faculty of Pharmacy, Assiut University, Assiut 71515, Egypt.
Abstract
Promising cytotoxic effects of several Gardenia species (Rubiaceae) have been established by many studies. The current study evaluated MTT-based cytotoxic activities of the crude extract from Gardenia thunbergia L. f. aerial parts and four fractions thereof, including n-hexane, dichloromethane (DCM), ethyl acetate, and aqueous, against human leukemia (HL-60) and hepatoma (HepG2) cell lines, as well as the normal (WI38) cell line. Both non-polar fractions, n-hexane and dichloromethane, showed tumor-selective toxicities against both tested cancerous cell lines. These results sparked our interest in chemically characterising these bioactive fractions to reveal their cytotoxic components. The composition of n-hexane-soluble fraction was investigated via GC-MS analysis, while column chromatographic separation was used to isolate the components of DCM-soluble fraction. These isolated phytochemicals were identified via spectroscopic analyses. Besides, the chemotaxonomic value of the detected phytochemicals and their reported cytotoxic profiles were discussed.
Keywords
Gardenia thunbergia; Rubiaceae; Cytotoxicity; Human leukemia; HepG2 cells; Chemotaxonomy
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