Assessment of expression profiles of three microRNAs and their clinical, laboratory, and histopathological correlations in non-obstructive azoospermia : A controlled study | ||
| Human Andrology | ||
| Article 1, Volume 12, Issue 12, April 2022, Pages 1-8 PDF (1.32 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ha.2021.111926.1083 | ||
| Authors | ||
| Rashad M Mostafa1; Hoda Y Abdallah2; Heba M Eissa3; Donia R Ibrahim4; Sherif M Ibrahim5; hany M Saad* 6 | ||
| 1Andrology department, Faculty of Medicine, SUEZ CANAL UNIVERSITY - FOM | ||
| 2Genetics Unit, Histology and Cell Biology Department, Faculty of Medicine, Suez Canal University | ||
| 3Dermatology Department, Elmenshawy General Hospital | ||
| 4Dermatology Department Port said General Hospital | ||
| 5Dermatology Department El Arish General Hospital | ||
| 6lecturer of andrology, faculty of medicine suez canal university, Ismalia, egypt | ||
| Abstract | ||
| Background: MicroRNAs (miRNAs) play essential roles in human spermatogenesis, but little is known about seminal plasma miRNAs in infertile men. Aim: To assess miRNA-34b, miRNA-181a, and miRNA-429 expression profiles in seminal plasma of patients with nonobstructive azoospermia (NOA) and to correlate these expression profiles with their clinical, laboratory, and histopathological features. Patients and Methods: This was a prospective case–control study which included two groups: 50 males with NOA and 50 healthy fertile males who attended the Andrology Outpatient Clinic, the Genetics Unit, and a private IVF Center. Semen analysis and assessment of the three miRNAs expression profiles in seminal plasma were done by real-time PCR for both groups. Hormonal profile assessment (follicle-stimulating hormone, luteinizing hormone, testosterone, and prolactin), testicular sperm extraction, and histopathology were done for the patients’ group. Results: In this study, there were statistically significant upregulations of both miRNA-429 and miRNA-181a in the patients’ group compared with controls (P<0.001), which was not the case for miRNA34b (P=0.259). There were statistically significant relationships between the three miRNAs, testicular sperm extraction results, and histopathological patterns (P<0.05).There were significant positive correlations between miRNA34b, miRNA-429, and follicle-stimulating hormone(r=0.466, P=0.001; r=0.375, P=0.009, respectively) and significant negative correlations between miRNA34b, miRNA-181a, and Johnsen’s score (r=-0.287, P=0.048; r=-0.351, P=0.015, respectively). There was statistically significant negative correlation between miRNA-429 and testosterone (r=-0.330, P=0.022). Conclusion: The expression levels of miRNA 181aandmiRNA-429 only were upregulated in NOA patients compared with controls. Thus, they may represent useful noninvasive biomarkers for NOA. | ||
| Keywords | ||
| Male infertility; microRNA-181a; microRNA-429; microRNA34b; nonobstructive azoospermia; spermatogenesis | ||
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