Hassab, A., El Shahat, M., Abbas, N., Khalil, H., Shamseya, M., Zaid, A. (2022). Potential Biotherapeutic Effect of Lactobacillus reuteri NA1 on The Treatment of Induced Peptic Ulcer in Rats: Histopathological Study. test, 4(1), 80-92. doi: 10.21608/jcvr.2022.240877
Alaa Hassab; Mohamed El Shahat; Nasser Abbas; Hany Khalil; Mohamed Shamseya; Anis Anis Mohamed Zaid. "Potential Biotherapeutic Effect of Lactobacillus reuteri NA1 on The Treatment of Induced Peptic Ulcer in Rats: Histopathological Study". test, 4, 1, 2022, 80-92. doi: 10.21608/jcvr.2022.240877
Hassab, A., El Shahat, M., Abbas, N., Khalil, H., Shamseya, M., Zaid, A. (2022). 'Potential Biotherapeutic Effect of Lactobacillus reuteri NA1 on The Treatment of Induced Peptic Ulcer in Rats: Histopathological Study', test, 4(1), pp. 80-92. doi: 10.21608/jcvr.2022.240877
Hassab, A., El Shahat, M., Abbas, N., Khalil, H., Shamseya, M., Zaid, A. Potential Biotherapeutic Effect of Lactobacillus reuteri NA1 on The Treatment of Induced Peptic Ulcer in Rats: Histopathological Study. test, 2022; 4(1): 80-92. doi: 10.21608/jcvr.2022.240877

Potential Biotherapeutic Effect of Lactobacillus reuteri NA1 on The Treatment of Induced Peptic Ulcer in Rats: Histopathological Study

Journal of Current Veterinary Research
Article 10, Volume 4, Issue 1, April 2022, Pages 80-92    PDF (1.54 M)
Document Type: Original Article
DOI: 10.21608/jcvr.2022.240877
Authors
Alaa Hassab1; Mohamed El Shahat1; Nasser Abbas1; Hany Khalil1; Mohamed Shamseya2; Anis Anis Mohamed Zaid* 3
1Biochemistry and Molecular Biology Department, Genetic Engineering and Biotechnology Researches Institute (GEBRI), University of Sadat City, Egypt.
2Department of clinical and experimental internal medicine, Medical Research institute, Alexandria University, Egypt.
3Department of Pathology, faculty of Veterinary Medicine, University of Sadat City, Egypt.
Abstract
         Peptic ulcer disease (PUD) is still a global public health problem. Development of novel therapies became necessary to overcome the limitations of traditional therapies. In this study we intend to treat experimentally induced peptic ulcer by using a new probiotic bacterial strain.Lactobacillus reuteri NA1 was isolated from peptic ulcer human-biopsy on MRS medium, molecularly identified, and registered in GenBank with accession number MH714546. This study was conducted on 2 experiments, the first experiment included 48 rats divided into 4 groups. Saline group, negative control group (saline); H.p. 1 group, H. pylori -ve stool administrated group; H.p. 2 group, H. pylori +ve stool administrated group; H.p. 3 group, H. pylori induced ulcer and treated with L. reuteri NA1. The second experiment included 36 rats divided into 3 groups. Saline group, negative control group (saline); As. 1 group, aspirin induced ulcer group; As. 2 group, aspirin induced ulcer and treated with L. reuteri NA1. Histopathological investigations showed a significant recovery of the experimentally induced gastric ulcers by both H. pylori and Aspirin after 6 weeks of the treatment with L. reuteri NA1. Complete healing of gastric mucosa was observed after 12 weeks of daily administration of L. reuteri NA1. L. reuteri NA1 has shown a promising solution for treatment of peptic ulcer in H. pylori- antibiotic resistance patients, and aspirin users.
 
Keywords
Peptic ulcer; Lactobacillus reuteri NA1; Probiotics; Helicobacter pylori; Histopathology
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