Genotyping of Rotavirus RNA by Sequencing among Children with Diarrhea at Zagazig University Hospital | ||
| The Egyptian Journal of Hospital Medicine | ||
| Article 66, Volume 88, Issue 1, July 2022, Pages 2710-2715 PDF (560.54 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejhm.2022.241123 | ||
| Authors | ||
| Elhamy Rifky abdelkhalik; Mohamed Abdelkader Adallah Almalky; Rania Mohamed Mohamed Ebrahim Amer; Hazem Ali Abd Elraouf | ||
| Abstract | ||
| Background: Worldwide, rotavirus (RV) is the most common cause of severe gastroenteritis among infants and young children. While diarrhea is the second most common cause of fatal childhood illness. RV can be detected in high concentrations in the stool of children suffering from gastroenteritis. Objectives: To determine rotavirus genotyping among diarrheic children at Sharkia Governorate. Patients and Methods: For one-year surveillance, the data and the stool samples were gathered from January 2015 to January 2016. A total of 140 stool specimens were collected from the inpatients diagnosed with acute diarrhea in the Pediatric Department, Zagazig University Hospitals. Results: Our results showed that there was a statistically significant difference between positive and negative rotavirus infection cases in Veslkari score of severity and hospital stay. 12.5% of the study group had G3 genotype and 33.6% of the study group had strain P8. By comparing clinical data between G3 and non-typeable genotyping in the studied group, there was a statistically significant difference between G3 and non-typeable genotyping in the Veslkari score of severity. There was a highly statistically significant negative correlation between the Veslkari score of severity and weight. On the other side, there was a statistically significant positive correlation between the Veslkari score of severity and age. Conclusion: We concluded that rotavirus represents a high percentage of hospitalized cases of GE in Zagazig University Hospital with a significant difference in severity and complications between positive and negative rota cases. | ||
| Keywords | ||
| Rotavirus RNA; Genotyping; Sequencing; Children; Diarrhea | ||
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