MICROPROPAGATION OF MALLING MERTON 106 APPLE ROOTSTOCK; I· SHOOT PROLIFERATION AND MULTIPLICATION | ||
| Journal of Plant Production | ||
| Article 14, Volume 28, Issue 3, March 2003, Pages 1969-1981 PDF (2.02 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jpp.2003.242495 | ||
| Author | ||
| M. B. EI-Sabrout, | ||
| Pomology Department, Faculty of Agriculture, AlexandriaU iverslty. Alexandria, Egypt. | ||
| Abstract | ||
| The present research was carried out during three successive ye rs (2000- 2002), in order to study the effect of Murashige and Skoog (1962) (MS) culture medium supplemented with various concenlrations of plant growth regula! rs cn lhe shoot prOliferation from shoot tip explants and sheet multiplication in Malfl g Merton 106 (MM106) apple rootstock cultures. The main results can be summarized In the forrowing points: I· Shoot proliferation percentage from shoot tip explant, average n mber of proliferated shoots per shoot tip explant and average length of proliferated shoot were slgnif1canUy the highest (96.67%, 7.79 and 2.36 cm, respectively) on MS medium + 2.0 mg1" SA + 0.2 mg1" NAA. 2- On MS medium + 1.0 mgr' SA + 0.5 mg1" Kin. the percentage of cultures wilh muttiple shoots from Original shoot. average number of new proliferat d shoots per original shoot and average length of new proliferated shoot were superior, during four successive subcultures. In the 1 II , 2nd , 3fd and 4'" subcultures. the percentage of cultures with multiple shoots from Original shoot was slgnirlcantly the highest (90.00%, 96.67%. 100.00% and 93.33%, respectively\. Average number of new prqlirerated shoots per original shoot was si9nificanlly the highest (7.52. 9.79.10.00 and 8.21, respectively), Average length of new proliferated shoot was sig ificantly the hi9hest (2,65cm, 2.83cm, 3.00cm and 2.7Ocm, respectively). | ||
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