PRODUCTION OF PIGEON PDX VACCINE FROM THE EGYPTIAN LOCAL STRAIN ON SPF EGGS | ||
| Egyptian Journal of Agricultural Research | ||
| Article 34, Volume 83, Issue 1, March 2005, Pages 429-438 PDF (2.35 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejar.2005.243339 | ||
| Authors | ||
| SHIRIN S. SAMER; AHMED M. DAOUD | ||
| Veterinary Serum and Vaccine Research Institute, Agricultural Research Centre, Ministry of Agriculture, Dokki, Giza, Egypt | ||
| Abstract | ||
| A local virulent Egyptian strain of pigeon pox virus was attenuated on specific pathogen free (SPF) eggs for production of pigeon pox vaccine. Twenty two serial passages for the virulent virus were applied on the chorioallantok membrane (CAM) of SPF eggs. Titration of the serial passages were recorded as 106.8, 107.5, 107.8, 108.5 and 108.5 EID50/m1 for the 5th, 10th, 15th, 18th and 20th passages, respectively. The pathogenicity of the 5th, 10th, 15th and 18th passages was tested by inoculation in susceptible pigeons, the 15th and 18th passages produced the most acceptable and mild post-vaodnal reaction. The 18th passage was considered the passage having the highest titre (108.5 EID50/m1). The quality control of the prepared vaccine was applied by testing sterility, safety and potency; the vaccine was proved to be sterile, safe and potent for pigeons which resisted the challenge with virulent pigeon pox virus. Confirmatory serological tests (SNT and EUSA) were applied and recorded that the antibody levels after vaccination reached the protective levels (more than 1.5 index for SNT and more than 1.0 for EUSA). | ||
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