ANTI-CANCER EFFECT OF SOME EGYPTIAN NATURAL PLANTS EXTRACTION AGAINST PROSTATE CANCER CELLS | ||
| African Journal of Biological Sciences | ||
| Articles in Press, Accepted Manuscript, Available Online from 12 June 2022 | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ajbs.2022.138129.1032 | ||
| Authors | ||
| Aysam M Fayed* 1; Amal M Abdelaziz2; khalid M Bassiouny3; Duaa M Hanafy4 | ||
| 1Molecular Biology Department , Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Egypt. | ||
| 2Department of Molecular Biology, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Egypt. | ||
| 3Department of Molecular Biology, Genetic Engineering and Biotechnology Research Institute,University of Sadat City, Egypt. | ||
| 4Department of Molecular Biology, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Egypt. | ||
| Abstract | ||
| Prostate cancer (PCa) is a malignant tumor that usually caused by many epigenetic alteration that lead to uncontrolled proliferation, differentiation and invasion to nearby tissues. Different types of treatment for patients of PCa have been occurred such as surgery, radiation, hormone and chemo-therapy. This work presented an experimental study for using low-cost and ecologically friendly Egypt plants’ extracts (garlic, cinnamon and liquorice) that have been dissolved in DEMSO. The plants extract were characterized by using different analytical means and techniques such as HPLC and GC-MS. To evaluate the potential effect of the selected extracts on, PC3 cells, MTT assay, qRT-PCR and ELISA techniques were studied. The results showed that the garlic exhibited a significant cytotoxic effect against PC3. Interestingly, the garlic was significantly increased the production of relative LDH in the treated cells rather than that were treated with cinnamon and liquorice extracts. Also, it was found that garlic components effects on pro‐apoptotic signaling including P53, Caspase-3 and modulates the Raf-1 signaling pathway, and down-regulating the RAF/MEK/ERK pathway in PC3 cells compared with cinnamon and liquorice extractions. ELISA results indicated that the mean concentrations of IL-1α and IL-6 increased dramatically after 24h. In conclusion, the garlic extract has an ability to adjust the secretion of pro inflammatory cytokines. Meanwhile, garlic based herbal can be used as effective and harmless anti-cancer agent for PCa treatment. | ||
| Keywords | ||
| Garlic; cinnamon; liquorice; P53; Raf-1 | ||
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