Phylogenetic relationship and systematic identification of different shrimp and prawn species in Egypt | ||
| Egyptian Journal of Aquatic Biology and Fisheries | ||
| Article 31, Volume 26, Issue 3, May and June 2022, Pages 565-578 PDF (1020.51 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejabf.2022.244300 | ||
| Author | ||
| Eman M. Abbas et al. | ||
| Abstract | ||
| Among the Egyptian catch fisheries, marine shrimp species represent the most economically important invertebrate. Likewise, this species is distributed all over the world as a diverse one. The current study merged DNA barcoding as a valuable complementary tool with morphology-based species identification. Ten different maritime shrimp species were phenotypically delineated and identified, based on the structure of the carapace; the crest, groove, and spines, the rostrum with its teeth; dorsally and ventrally, sex determination; the shape of the petasma and thelycum, armed or unarmed telson, and the body colour. Phylogenetic relationships between ten Egyptian shrimp species were examined with the nucleotide sequence data stored in the GenBank database of partly region for the mitochondrial subunit I of cytochrome oxidase gene (COI). Most of the produced sequences had 99–100% similarity to conspecific sequences. Sequence assessment of the COI gene for the phylogenetic tree distinguished all ten shrimp species into three definite clades, which are genetically distinct from one another and demonstrated the same phylogenetic reservations to their respective genus. The percent GC content ranged between 41.4% in Metapenaeus monoceros and 36.6 % in Trachypenaeus curvirostris and Marsupenaeus japonicas. The highest genetic distance (0.3) was found between Palaemon serratus and Parapenaeus longirostris of Egypt, and Penaeus semisulcatus of India and Trachypenaeus curvirostris of Egypt. The lowest genetic distance (0.0) was observed between Parapenaeus longirostris of Egypt and Turkey, and Xiphopenaeus kroyeri of Egypt, Mexico, and the USA, overlapping each other. The current study clearly shows that DNA barcoding may be used for the differentiation between shrimp species, which will help researchers better understand the biology of evolution and conservation. | ||
| Keywords | ||
| DNA barcoding; COI gene; Morphology; Phylogeny; Shrimp species | ||
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