MOLECULAR FINGERPRINT OF SOME NEW HOT PEPPER "Capsicum annuum L.tI CULTIVARS IN EGYPT | ||
| Journal of Plant Production | ||
| Article 28, Volume 28, Issue 2, February 2003, Pages 1311-1326 PDF (9.99 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jpp.2003.245532 | ||
| Author | ||
| Mahassen M. A. Sidky | ||
| Medicinal and Aromatic plant Research Department, Hortlculture Research Institute, Agricultural Research Center, Dokky, Cairo, Egypt. | ||
| Abstract | ||
| The present investigation was conducted to develop biochemical and molecular genetic fingerprints for the four Capsicum annuum cultivars: Bohemian, Cherry, Long· Red Cayenne and Anaheim, Polyacrylamide gel electrophoresis was employed to identify the biochemical fingerprint based on SOS·proteins (sodium dodecyl sulfate) and two isozyme (peroxidase and esterase) profiles, The gained data of 50S-protein patterns revealed low level of polymorphism whereas the two isozyme systems were effective in identifying Ihe four Cap < /em>sicum cultivars. Each cultivar was distinguished by a unique class pattern, which indicates that isozyme fingerprinting is a reliable technique to discriminate the four Capsicum cultivars. Extracted DNA from fresh leaf samples was used to identify the molecular fingerprint of the four cultivars of Capsicum under study. Randomly amplified polymorphic DNA-polymerase chain reaction (RAPO-peR) analysis with frye random primers (UBC-20, -35, -50, OPS-08 and -09) was performed. The results of RAPD analysis partial,. discriminate among the four cullivars of Capsicum by one or more unique bands. Some of Ihe random primers were more successful in identrfying cultivars (USC-20) than the others, where they generated high number of unique bands, The similarity indices for all pairs of four groups of Capsicum cultivars scored from polymorphism across 50S-protein, two isozymes and RAPD analysis were used to identify the genetic relatlonships among these cultivars, Keywords: Capsicum, pepper, electrophoresis, lsozymes, RAPO-PCR, similarity, INTRODUCTION Genetic fingerprinting studies of Cap < /em>sicum cultivars offer possibilities for use in classifying and improving Capsicum cultivars. SOS-polyacrylamide gel electrophoresis of proteins, isozymes and PCR-RAPO (polymerase chain reacuons-randornly amplified polymorphic DNA) analyses offer reliably rapid means for establishing genetic profiles and elucidation of genetic relationships within and between taxa that would help in conservation of new varieties and save efforts, expanses and time in the breeding programs of Capsicum. Badr (1995) used SDS-protein profiles to study the relationships between some taxa of Trifolium. Saeed et ai, (1998) used SDS protein profiles to discriminate between two jujube cultivars. Afiah at al. (1999) studied the protein profiles of some wheat cultivars and their F, hybrids. Also Aly et al. (2000) used grain storage protein to discriminate between nine rice cullivars. Isozymes have been extensively utilized to deduce similarity indices and to construct dendrogram trees in many plant taxa. In this regard, Abdelsalam et a/. (1998) used isozymes to discriminate between some barley cultivars. Also, Li and Li (1999) used 9 isoenzyme systems to investigate variation in twelve natural populations of Eucalyptus microtheca from Australia. Cluster analysis based on the UPGMA (un-weighted pair group method of arithmetic means) dendrograrn revealed low levels of genetic | ||
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