POTENTIALITY OF Quercus robur, L. FOR INDUCING SHOOTLETS MASS PRODUCTION THROUGH TISSUE CULTURE TECHNIQUES AND FACTORS INFLUENCING ITS SUCCESS | ||
| Journal of Soil Sciences and Agricultural Engineering | ||
| Article 4, Volume 28, Issue 2, February 2003, Pages 1531-1544 PDF (2.88 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jssae.2003.246115 | ||
| Authors | ||
| Mervat M. Gad,; M. S. Shehata | ||
| Forestry and Woody Trees Dept., Horticultural Research Institute, Agricultural Research Center, Giza, Egypt | ||
| Abstract | ||
| This work was investigated in order to oblain mass production of snoouets from Q. robur, as a promising tree. Ph.,,1CI exudates was a major problem, which was overcome by manipulating the mother seedling by a dark system followed by handling the explants obtained by successive steps. The effect of explant position on mother seedling branch, medium type, plant growth regulators (type and concentration), sucrose concentration, and the solidifying agent (agar and gerlite), were Investigated. Favourable culture establishment was obtained by Inoculating the medial nodes segments on WPM supplemented with 0.2 mg L" ISA + 0.8 mg L" SA rather than on QL medium. A high frequency of shooUets multiplication (60/explant) was obtained on WPM supplemented with 3% sucrose and 0.2 eng L'! ISA + 1.2 mg L·1 SA. Successful rooting of shooUets was induced by modifying WPM with 0.5 mg L" IBA and solidified by 0.2% genite which surpassed 0.7% agar in rooting criteria. | ||
| Keywords | ||
| Quercus robur; tissue culture; phenol exudates; explant position; gelling agent; mother plant | ||
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