Histological and Immunohistochemical Study on the Effect of the Combined Vitamin C and Metformin versus Metformin Alone in Treating Diabetic Myopathy in Rat | ||
| Journal of Medical Histology | ||
| Article 6, Volume 5, Issue 1, June 2021, Pages 75-87 PDF (1.46 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jmh.2021.98961.1093 | ||
| Author | ||
| Hanaa Mousa* | ||
| Department of Medical Histology and Cell Biology, Faculty of Medicine, Zagazig University, Egypt | ||
| Abstract | ||
| Background: Metformin is used in type 2 diabetes mellitus (T2DM). Vitamin C is a water-soluble vitamin with antioxidant and anti-inflammatory properties. Therefore, the effect of combining metformin and vitamin C in treating the diabetic myopathy was investigated in this research. Methods: Forty rats were used divided into 4 groups; control group (group I), diabetic group (group II), metformin group < br />(group III), and vitamin C and metformin group (group IV). Body weight was measured before and at the end of the experiment. Blood glucose levels and serum C-reactive protein (CRP) were measured. Tissue malondialdehyde (MDA) and superoxide dismutase (SOD) were measured. Sections of the skeletal muscle were stained with H&E, Masson trichrome, and caspase-3 and inducible nitric oxide synthase (iNOS) immnuostaining. Morphometric and statistical analyses for the results were performed. Results: Body weight of rats of group I and group II were decreased then increased in group IV. Blood glucose levels were elevated in group II; and reduced in group III and group IV. MDA level were increased in group II, then lowered in both in group III and group IV.SOD activity was decreased in group II, then increased in group III and group IV toward normal level. Collagen deposition and immune reaction to caspase-3 were elevated in group II and group III and group IV. Immune reaction to iNOS was elevated in group II and group III, and nearly the same as group I in group IV. Conclusions: Combined intake of vitamin C and metformin provided better results in treating diabetic myopathy than that obtained from metformin alone. | ||
| Keywords | ||
| Diabetic myopathy; Inflammation; Metformin; Oxidative stress; Vitamin C | ||
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