Detection of rmpA and magA genes in Hypervirulent Klebsiella pneumoniae Isolates from Tertiary Care Hospitals | ||
| Egyptian Journal of Medical Microbiology | ||
| Volume 31, Issue 3, July 2022, Pages 43-50 PDF (357.26 K) | ||
| Document Type: New and original researches in the field of Microbiology. | ||
| DOI: 10.21608/ejmm.2022.247186 | ||
| Authors | ||
| Mona Adel Khattab* 1; Raghda Hager2 | ||
| 1Medical Microbiology & Immunology Faculty of Medicine Ain Shams University | ||
| 2Medical Microbiology &Immunology Faculty of Medicine, Misr University for Science and Technology | ||
| Abstract | ||
| Background: Hypervirulent Klebsiella pneumoniae (hvKp) cause multiple dangerous infections. HvKp can be distinguished from classical K. pneumoniae (cKp) by hyper-mucoviscosity. Presence of multiple genes contribute to hypervirulent phenotype of K. pneumoniae as mucoviscosity-associated gene A (magA) and regulator of mucoid phenotype A (rmpA). Objectives: to detect hvKp frequency among strains of k.pneumoniae by using string test phenotypically, to use multiplex polymerase chain reaction for rmpA and magA genes detection. Methodology: Fifty isolates of k.pneumoniae, were obtained from hospitalized patients in El-zohour Hospital. Conventional identification and susceptibility to antimicrobials were performed. Results: Most strains were isolated from samples of urine. Thirty strains out of fifty (60%) were hvKp by using string test. magA and rmpA were present in 14 isolates (28%) and 29 isolates (58.0%) respectively among k.pneumoniae isolates. Out of thirty hvKp strains, magA and rmpA genes were present in 14 stains (46.7%) and 25 strains (83.3%) respectively and 12 strains (40%) were coharboring both genes and were string test positive. Conclusions: high frequency of hvKp among clinical samples. Prompt detection of hvKp in different clinical settings is needed through establishing effective diagnostic tools. String test is a sensitive tool for rmpA gene detection. Establishment of both proper preventive measures and antimicrobial stewardship programs may be helpful to overcome spreading of multidrug resistant hvKp. | ||
| Keywords | ||
| Hypervirulent k. pneumonia (hvKP); string test; mucoviscosity-associated gene A (magA) and regulator of mucoid phenotype A (rmpA) | ||
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