Cytogenetic and Pathological Studies on The Effect of Gibberellic acid in rabbit | ||
| Egyptian Journal of Chemistry and Environmental Health | ||
| Volume 2, Issue 2, November 2016, Pages 566-579 PDF (829 K) | ||
| Document Type: Scientific and Research | ||
| DOI: 10.21608/ejceh.2016.248061 | ||
| Authors | ||
| M. Abdou I; Mervat A Ayoub* ; Maha M El Aalem* | ||
| Biochemistry and Pathology Dept., Animal Health Research Institute, Zagazig Lab | ||
| Abstract | ||
| This study was designed to clarify the effect of the plant growth hormone, gibberellic acid (GA3) on the cytogenetic and pathological profile of adult male rabbits. Thirty bucks were classified into 3 equal groups; a control and two other treated groups; the first (indirectly exposed group, IEG) was forced to feed on a previously sprayed green fodder (alfalfa) with the recommended dose of GA3, while the second (directly exposed group, DEG) was forced to drink ad libitum on 75 ppm GA3 in water for 30 successive days. Both exposed groups evoked a significant increase in the total aberrated cells and total chromosomal aberrations of bone marrow cells, however, the most predominated chromosomal aberrations were deletions, ring chromosomes, end to end associations and aneuploidy, in addition to DNA damage using comet test. The second treated group (DEG) showed a significant increase in the percentage of these aberrations not only comparing to the control but also with the first treated group (IEG). Pathological changes in the liver, kidneys, lungs and testes were reported in both treated groups. These changes were severe in the second treated group (DEG). After one month recovery period, the deviated parameters of the first treated group (IEG) were nearly returned to the normal values, meanwhile, the second treated group (DEG) still revealed significant changes compared to either the control or the first treated group (IEG). From this study, It could be concluded that GA3 has a genotoxic and cytotoxic effects. These effects were severe in rabbits directly exposed to the hormone (DEG) compared to those fed on sprayed green fodder with its recommended dose (IEG). | ||
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