Validation of HPLC Method for Determination of Doxycycline hyclate in turkey serum | ||
| Egyptian Journal of Chemistry and Environmental Health | ||
| Volume 2, Issue 2, November 2016, Pages 282-291 PDF (422.74 K) | ||
| Document Type: Scientific and Research | ||
| DOI: 10.21608/ejceh.2016.248064 | ||
| Authors | ||
| Nahla S. Elshater* 1; Fatma Hussein1; Mai A Fadel1; Marwa Ragab1; Heba Hassan1; Maha Sabry2 | ||
| 1Reference Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute,Dokki | ||
| 2Chemistry Department, Animal Health Research Institute,Dokki | ||
| Abstract | ||
| An accurate, sensitive and reproducible high performance liquid chromatographic (HPLC) method for the quantitation of doxycycline in turkey serum has beendeveloped and validated. The doxycycline standard was eluted from a C18 column (4.6 mm i.d., 250 mm, 5 µm particle size) at 15 ⁰C with a mobile phase consisting of 5% acetic acid : methanol : acetonitrile (55:20:25, v/v/v). A UV detector set at 347 nm was used to monitor the effluent. Retention time of doxycycline was approximately at 4 min and the run time was 10 min. The column temperature adjusted at 15 ⁰C with a flow rate 1ml/min and injection volume 25 µl. Doxycycline was extracted from serum by ACN. Linearity was confirmed in the concentration range 0.1-2 µg/ml, had regression coefficients (R2) 0.9999 for doxycycline quantification in turkey serum. The analytical conditions gave a recoveries in a range of 97.57% to 99.83%. The limit of detection (LOD) was 0.035 µg/ mL and limit of quantification (LOQ) was 0.108 µg/ml. The relative standard deviation (RSD %) was less than 2% in all cases. The developed method was accurate, precise and rapid for simultaneous estimation of doxycycline in turkey serum samples. | ||
| Keywords | ||
| Validation; Doxycycline; Serum; HPLC | ||
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