Detection of Enterococcal asa1 and vanA Genes in Clinical Samples from Adult Immunocompromised Patients | ||
| Egyptian Journal of Medical Microbiology | ||
| Volume 29, Issue 1, January 2020, Pages 133-138 PDF (310.11 K) | ||
| Document Type: New and original researches in the field of Microbiology. | ||
| DOI: 10.21608/ejmm.2020.249870 | ||
| Authors | ||
| Nesreen M. Kamel* 1; Mai E. Ahmed1; Mohamed N. Salem2; Ahmed F. Azmy3; Ehab M. Fahmy4 | ||
| 1Department of Clinical Pathology | ||
| 2Department of Internal Medicine Faculty of Medicine – Beni-Suef University, Beni-suef, Egypt | ||
| 3Department of Microbiology, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, Egypt | ||
| 4Department of Medical Microbiology and Immunology, Aswan Faculty of Medicine, Aswan, Egypt | ||
| Abstract | ||
| Background: Vancomycin resistant Enterococci (VRE) is important opportunistic nosocomial pathogens due to rapid spread, and limited treatment options. Objectives: The aims of this study were to assess incidence of enterococcal infections and VRE in adult immunocompromised patients and detection of asa1 and vanA genes among these isolates. Methodology: The study included 80 adult immunocompromised patients admitted to Beni-Suef University Hospital. Antimicrobial susceptibility was done by disc diffusion method then MIC was done for vancomycin resistant strains and Enterococcus was considered VRE if the MIC was ≥ 16μg/ml. PCR was done for detection of vanA and asa1 genes. Results: Enterococci were detected in 36 samples, 22.2% of isolates were vancomycin resistant. vanA and asa1 were detected in 33.3% and 63.8% of isolates respectively. VanA gene was detected in 4 (50%) of VRE whereas vanA gene was detected in 4 VSE isolates. Comclusion: Further studies are needed for detection of other virulence and resistance genes and their role in bacterial pathogenicity. | ||
| Keywords | ||
| Immunocompromised; Enterococci; VRE; vanA; asa1 | ||
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