The Diversity of Gut Microbiota among Type 1 and Type 2 Egyptian Diabetic Patients | ||
| Egyptian Journal of Medical Microbiology | ||
| Volume 29, Issue 2, April 2020, Pages 123-130 PDF (371.23 K) | ||
| Document Type: New and original researches in the field of Microbiology. | ||
| DOI: 10.21608/ejmm.2020.250216 | ||
| Authors | ||
| Nagwan G. El Menofy* ; Hend M. Radwan; Sahar M.R. Radwan | ||
| Microbiology and Immunology Department, Faculty of Pharmacy (Girls), Al-Azhar University, Cairo, Egypt | ||
| Abstract | ||
| Background: Gut microbiome could have central role in development of type 1 and type 2 diabetes mellitus (T1D) & (T2D). Objectives: The aim of the present study is to detect the composition of gut microbiota among T1D and T2D patients compared to healthy individuals. Methodology: The intestinal microbial composition of diabetic and heathy groups was investigated by both traditional culture techniques and polymerase chain reactions with amplification of the 16-23S rRNA intergenic spacer (IS) region. Results: By applying culture, diabetic groups showed a non-significant higher Firmicutes/ Bacteroidetes ratio (4.7% in T1D and 9% in T2D) compared to control group (4%) P value is 0.2058. While, PCR method showed that the diabetic group had a non-significant higher percentage of Lactobacillus spp., Faecalibacterium prausnitzii, Alistipes spp., Bacteroides thetaiotaomicron and Akkermansia muciniphila compared to healthy group (P value=0.407). Conclusion: Both diabetic groups showed a non-significant Firmicutes/Bacteroidetes ratio compared to control group by both culture and PCR techniques. | ||
| Keywords | ||
| Microbiota; Microbiome; Type 1 diabetes; Type 2 diabetes; Intergenic spacer | ||
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