Elkhyat, A., Makled, A., Albeltagy, A., Keshk, T., Dawoud, A. (2020). Prevalence of vanA Gene among Methicillin Resistant S. aureus Strains Isolated from Burn Wound Infections in Menoufia University Hospitals. test, 29(3), 97-104. doi: 10.51429/EJMM29313
Amira H. Elkhyat; Amal F. Makled; Ahmed M. Albeltagy; Tarek F. Keshk; Amal M. Dawoud. "Prevalence of vanA Gene among Methicillin Resistant S. aureus Strains Isolated from Burn Wound Infections in Menoufia University Hospitals". test, 29, 3, 2020, 97-104. doi: 10.51429/EJMM29313
Elkhyat, A., Makled, A., Albeltagy, A., Keshk, T., Dawoud, A. (2020). 'Prevalence of vanA Gene among Methicillin Resistant S. aureus Strains Isolated from Burn Wound Infections in Menoufia University Hospitals', test, 29(3), pp. 97-104. doi: 10.51429/EJMM29313
Elkhyat, A., Makled, A., Albeltagy, A., Keshk, T., Dawoud, A. Prevalence of vanA Gene among Methicillin Resistant S. aureus Strains Isolated from Burn Wound Infections in Menoufia University Hospitals. test, 2020; 29(3): 97-104. doi: 10.51429/EJMM29313

Prevalence of vanA Gene among Methicillin Resistant S. aureus Strains Isolated from Burn Wound Infections in Menoufia University Hospitals

Egyptian Journal of Medical Microbiology
Volume 29, Issue 3, July 2020, Pages 97-104    PDF (438.32 K)
Document Type: New and original researches in the field of Microbiology.
DOI: 10.51429/EJMM29313
Authors
Amira H. Elkhyat* 1; Amal F. Makled1; Ahmed M. Albeltagy2; Tarek F. Keshk3; Amal M. Dawoud1
1Department of Medical Microbiology and Immunology, Faculty of Medicine, Menoufia University, Egypt
2Department of Clinical Pathology, Faculty of Medicine, Mansoura University, Egypt
3Department of Plastic Surgery, Faculty of Medicine, Menoufia University, Egypt
Abstract
Background: Staphylococcus aureus is a leading cause of burn wound infection. Vancomycin resistance among methicillin-resistant Staphylococcus aureus (MRSA) is becoming a worldwide growing threat. Objectives: to detect the prevalence of MRSA in burn patients and its antibiotic susceptibility patterns. In addition, the resistance patterns of MRSA to vancomycin and the prevalence of vanA gene among MRSA isolates were investigated. Methodology: A total 250 clinical samples were obtained from patients admitted to Burn Unit in Menoufia University Hospitals. Identification and antimicrobial susceptibility testing of S. aureus isolates were performed. Cefoxitin disk diffusion method was used to identify MRSA strains. Vancomycin resistance was determined by agar dilution method. Detection of mecA and vanA genes by multiplex PCR was done. Results: Staphylococcus aureus represented 43.3% of all isolates. By cefoxitin disc diffusion method, 94% (79/84) of isolated S. aureus were MRSA that showed a high resistance to most antimicrobials used with rates ranged from 40.5 % to 100%. Phenotypically among MRSA isolates, vancomycin sensitive S. aureus (VSSA), vancomycin-intermediate S. aureus (VISA) and vancomycin-resistant S. aureus (VRSA) were 59.5%, 15.2%, 25.3% respectively. Among MRSA isolates, 17 (21.5%) isolates had vanA gene by PCR (16 isolates were VRSA and one isolate was VSSA).Conclusion: This study is considered as an alarm demonstrating that implementation of proper infection control measures is mandatory to control spread of such resistant strains in our hospital.
Keywords
Vancomycin resistant Staphylococcus aureus; MRSA - mecAgene -vanAgene
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