Fahmy, N., Ahmed, N., Galal, G., Abdel Elmoaty, M., Abudeif, A., Kadry, Z., Mohamed, N. (2020). Study the Role of Tp53 in the Development of Hepatocellular Carcinoma in HCV Infected Patients. test, 29(4), 165-172. doi: 10.51429/EJMM29421
Nahed F. Fahmy; Nagwa S. Ahmed; Ghada Galal; Mohamed A. Abdel Elmoaty; Ahmed Abudeif; Zeinab M. Kadry; Nesma A. Mohamed. "Study the Role of Tp53 in the Development of Hepatocellular Carcinoma in HCV Infected Patients". test, 29, 4, 2020, 165-172. doi: 10.51429/EJMM29421
Fahmy, N., Ahmed, N., Galal, G., Abdel Elmoaty, M., Abudeif, A., Kadry, Z., Mohamed, N. (2020). 'Study the Role of Tp53 in the Development of Hepatocellular Carcinoma in HCV Infected Patients', test, 29(4), pp. 165-172. doi: 10.51429/EJMM29421
Fahmy, N., Ahmed, N., Galal, G., Abdel Elmoaty, M., Abudeif, A., Kadry, Z., Mohamed, N. Study the Role of Tp53 in the Development of Hepatocellular Carcinoma in HCV Infected Patients. test, 2020; 29(4): 165-172. doi: 10.51429/EJMM29421

Study the Role of Tp53 in the Development of Hepatocellular Carcinoma in HCV Infected Patients

Egyptian Journal of Medical Microbiology
Volume 29, Issue 4, October 2020, Pages 165-172    PDF (307.42 K)
Document Type: New and original researches in the field of Microbiology.
DOI: 10.51429/EJMM29421
Authors
Nahed F. Fahmy* 1; Nagwa S. Ahmed2; Ghada Galal3; Mohamed A. Abdel Elmoaty2; Ahmed Abudeif3; Zeinab M. Kadry2; Nesma A. Mohamed1
1Microbiology & Immunology Department, Faculty of Medicine, Sohag University, Egypt
2Biochemistry Department, Faculty of Medicine, Sohag University, Egypt
3Tropical Medicine Department, Faculty of Medicine, Sohag University, Egypt
Abstract
Background: Hepatocellular carcinoma (HCC) is considered as the primary malignancy of the liver that usually occurs on top of chronic viral hepatitis. The TP53 (tumor protein 53) is a tumor suppressor gene which is the key in tumor development and progression and the single nucleotide polymorphism (SNP) of the p53 gene codon 72 (p53Arg/Pro) changes the structure of the protein. Objectives: to determine the association of the TP53 Arg72Pro polymorphism with the risk of HCC development in Hepatitis C virus (HCV) infected Egyptian patients. Methodology: This is a case control study conducted in Sohag University Hospital on 100 participants (20 HCC, 20 CHCV, 40 LC and 20 control). TP53 gene polymorphism was identified by Polymerase chain reaction-Restriction fragment length polymorphism (PCR-RFLP), serum level of TP53 was measured by quantitative ELISA. Results: The GG genotype / G allele has the highest frequency in the controls, while the CC genotype/ C allele was more frequently found in the HCC patients. Carriage of TP53 CC genotype and TP53 serum level have a statistical significant association with HCC development. Conclusion: the CC genotype and the Pro (C) allele of TP53, codon 72 are risk factors for HCC in patients infected with HCV and the serum TP53 level is overexpressed in HCC patients.
Keywords
Tumor protein 53; Hepatocellular carcinoma; Hepatitis C virus
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