PRODUCTION OF SALT-TOLERANT SOUR ORANGE PLANTS (Citrus aurantium L.) BY USING TISSUE CULTURE TECHNIQUE: IV-MICROPROPAGATION OF SALT-TOLERANT PLANTLETS | ||
| Journal of Plant Production | ||
| Article 21, Volume 27, Issue 3, March 2002, Pages 1735-1748 PDF (595.22 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jpp.2002.253749 | ||
| Authors | ||
| A. M. El-Gazzar,; M. B. El-Sabrout | ||
| Department of Pomology, Faculty of Agriculture, Alexandria University, Alexandria, Egypt. | ||
| Abstract | ||
| This study was conducted through 1996-1999 years in the Department of Pomology, Faculty of Agriculture, Alexandria University. This work involved the development of an efficient protocol for in vitro propagation of salt tolerant plantlets of sour orange from shoot tips excised from the fully grown embryoids (obtained by somatic embryogenesis). The main results can be summarized in the following points: 1-On the MS (1962) medium containing 1mgl-1 BAP, 0.5mgl-1 kinetin and 0.5mgl-1 NAA, the percentage of cultures with multiple shoots was significantly the highest (80%).The average number of proliferated shoots was significantly the highest (6.798).The average length of proliferated shoot was significantly the highest (2.64 cm). 2- The MS (1962) medium containing 0.25 mgl-1 BAP + 0.5 mgl-1 NAA +0.5 mgl-1 IBA resulted significantly the highest rooting percentage of proliferated shoots (80.00%). Also, on the same medium, the average number of roots per proliferated shoot was significantly the highest (4.84). The average length of root per proliferated shoot was significantly the highest (1.6cm). 3- Eighty percent of the salt – tolerant plants of sour orange were successfully transplanted in soil. These plants survived, grew vigorously and showed no damage at 10gl-1 NaCl compared to the control plants (derived from NaCl sensitive callus) which showed sever leaf burn, progressive wilting and decreased growth at 2gl-1 NaCl. | ||
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