Chemical compositions of chicken eggs following infection with Infectious bronchitis virus | ||
| Egyptian Journal of Chemistry and Environmental Health | ||
| Volume 1, Issue 1, November 2015, Pages 821-836 PDF (665.94 K) | ||
| Document Type: Scientific and Research | ||
| DOI: 10.21608/ejceh.2015.253912 | ||
| Authors | ||
| Bayoumie HA A* 1; El Nile M. B.2; L. K. Abd El-Samie3; Maha M El Deib4 | ||
| 1Senior Researcher, Animal health Res. Inst. Zagazig (Poult .Dis. Dept.) | ||
| 2Researcher, Animal health Res. Inst. Zagazig (Chemistry. Dept.) | ||
| 3Assistant consultant of Poult. And Rabbit Dis. (Vet. Hospital) Facult. Vet. Med. Zagazig Univ | ||
| 4Colleague of Bio chem. (central lab.) Facult. Vet. Med. Zagazig Univ | ||
| Abstract | ||
| IBV detection and isolation trials was done to set its relationship to the inner egg changes.Rapid hemagglutination (HA) activity after neuraminidase enzyme treatment of the concentrated allantoic fluid (AF) of inoculated embryonating chicken eggs (ECE) can give a positive indication for the presence of IBV. The specificity of rapid HA test was examined with a non-hemagglutinating avian viruses such as infectious bursal disease virus (IBDV). The sensitivity of the test was compared with reversed transcriptase-polymerase chain reaction. The results showed that this test was specific and had a sensitivity of 100% for IBV detection. The detected IBV strain from Sharkia governorate was examined molecularly using polymerase chain reaction (PCR) and S-l partial gene sequence. Sequencing showed that this isolate is an IBV variant 2 that resembles the Egyptian IBV strain (Eg /12120 S/2012 and IS/1494/2006) field strains with 99% identity. The isolated virus designated (IBV-EG/ SHARKIA – F-629-2015) had showed (85.6%) similarity to the 4/91 variant vaccine, and (82.9 %) similarity to Dutch variants D-274 vaccinal strain, beside (82.2%) similarity to the classical vaccinal strains M-41. MA-5, H120. In the present study the following parameters were investigated (Total Lipid, CholesteroL, Triglcerol, Phospholipids, NEFA, MDA, Albumin protein, Yolk protein and Whole protein beside Calcium, Phosphorus, Magnesium, Manganese, Potassium, Chloride and PH ) Chemical analysis of egg content may explain that deformed eggs had resulted from inappropriate shell deposition on an unstable watery albumen base helped by the contractility of the oviduct due the disturbance in sodium and potassium pump . Watery albumen had resulted from an increase in PH and changes in sodium, potassium and chlorine concentrations, which leads to massive chemical changes in egg white and yolk. As far we know. This is the first attempt to study the impact of IBV infection on chicken egg biochemical composition | ||
| Keywords | ||
| IBV; rapid HA; neuraminidase enzyme treatment for IBV; PCR; Sequencing; Egyptian IBV variant 2 | ||
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