Prevalence, phenotypic characterization of listeria monocytogenes isolated from diseased sheep | ||
| Suez Canal Veterinary Medical Journal. SCVMJ | ||
| Articles in Press, Accepted Manuscript, Available Online from 10 August 2022 | ||
| Document Type: Original Article | ||
| DOI: 10.21608/scvmj.2022.145088.1087 | ||
| Authors | ||
| Mahmoud Ezzat1; marwa ibrahim2; Attia ELgedway3; Reham Mokhtar ELTarabili* 4; marwa arnout5 | ||
| 1Professor of Microbiology, Faculty of Veterinary Medicine, Suez Canal University | ||
| 2microbiology department,faculty of veterinary medicine,Zagazig university | ||
| 3Microbiology Department, Animal Health Research Institute ,Dokki ,Egypt | ||
| 4bacteriology,immunoloy and mycology department, faculty of veterinary medicine , Suez Canal University, Ismailia, Egypt | ||
| 5veterinary quarintine,Cairo airport | ||
| Abstract | ||
| Listeriosis is the third most common foodborne illness and it is considered one of the most dangerous bacterial zoonotic infections globally. This research aimed to isolate and characterize Listeria monocytogenes (L. monocytogenes) from diseased sheep in Egypt. A total of 240 samples collected aseptically from sheep were subjected to isolation and biochemical characterization of L. monocytogenes. L. monocytogenes, which are Gram-positive rods that are both aerobic and facultatively anaerobic. Thirty-one listeria isolates (12.9%) were recovered from diseased sheep. All tested isolates were positive for catalase, Voges-Proskauer, carbohydrate fermentation, esculin, gelatin hydrolysis and methyl red tests. Meanwhile, they were negative for oxidase, indole, urease and nitrate reduction tests and they did not utilize citrate in the citrate utilization test. Listeria isolates produced yellow butt/yellow slant on TSI agar medium with no H2S or gas production. Therefore, this work focused on isolation of L. monocytgenes from diseased sheep and biochemical characterization of the recovered isolates using traditional and Microbact™ 12L listeria identification system. | ||
| Keywords | ||
| Listeria monocytogenes; sheep; phenotypic identification; biochemical tests | ||
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