HERITABILITY AND RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) OF SOME EXOTIC BARLEY (Hordeum vulgare L.) GENOTYPES UNDER RAS SUDR CONDITIONS SOUTH SINAI | ||
| Journal of Plant Production | ||
| Article 14, Volume 27, Issue 4, April 2002, Pages 2173-2183 PDF (3.01 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jpp.2002.253973 | ||
| Authors | ||
| A. I. A. Hassan,; Nahed A. K. Rashed | ||
| Plant Genetic Resources Dept., Desert Research Center | ||
| Abstract | ||
| Four experiments were carried out at Ras Sudr Agricultural Research Station of Desert Research Center during the two successive seasons of1998/1999 and 1999/2000 to estimate the heritability values for yield and yield attributes for the four exotic barley (Hordeum vulgare L.) genotypes i.e. 1) ICD-84-0838-IAP-OTR, 2) CD 29706-E-Sy-3M-IY-IB-OAP, 3) L93-6AP-IAP-IAP-OAP and 4) APM-Hc-ISIS- 41/Rabour/3 Arar. In addition, random amplified polymorphic DNA (RAPD) analysis was used for the assessment the genetic diversity and genetic similarity between the studied barley genotypes. Heritability estimations under the two saline water irrigation and salinity levels were generally high for all studied traits [No. of spikes 1 plant, No. of grainsl spike, 1000-grain weight, grain yield 1 plant and harvest index (%) 1 which ranged from 40% for No. of grains 1 spike to e4% for No. of spikes 1 plant at low irrigation water salinity level. Six random 10-mer primers were used to amplify DNA via polymerase chain reaction (PCR) and 75 RAPDs were generated the six detected polymorphism in all four barley genotypes. Out of the total amplified bands, 72% of which were polymorphic. Cluster analysis by the unweighted pair group method of arithmetic means (UPGMA) showed that the genotypes; 4 and 3 were grouped together with similar coefficient 84%. Genotype 2 was the least similar barley genotype that its similarity ranged from 49% -56% with the rest of all genotypes studied, also, it attained the lowest heritability value. The results obtained could be used for selection the promising parents to generate an effective breeding barley program under saline condition. | ||
| Keywords | ||
| Hordeum vulgare L; Exotic barley lines; RAPD; Genetic similarity; Genetic diversity; Stress conditions | ||
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