Aslan Çelik, B., Ayan, A., Çelik, &., Yılmaz, A., Orunç Kılınç, &., Oktay Ayan, &. (2022). Molecular Identification of Hepatozoon canis in Ticks from Dogs in Siirt, Turkey. test, 53(4), 541-546. doi: 10.21608/ejvs.2022.146290.1355
Burçak Aslan Çelik; Adnan Ayan; Özgür Yaşar Çelik; Ali Bilgin Yılmaz; Özlem Orunç Kılınç; Özge Oktay Ayan. "Molecular Identification of Hepatozoon canis in Ticks from Dogs in Siirt, Turkey". test, 53, 4, 2022, 541-546. doi: 10.21608/ejvs.2022.146290.1355
Aslan Çelik, B., Ayan, A., Çelik, &., Yılmaz, A., Orunç Kılınç, &., Oktay Ayan, &. (2022). 'Molecular Identification of Hepatozoon canis in Ticks from Dogs in Siirt, Turkey', test, 53(4), pp. 541-546. doi: 10.21608/ejvs.2022.146290.1355
Aslan Çelik, B., Ayan, A., Çelik, &., Yılmaz, A., Orunç Kılınç, &., Oktay Ayan, &. Molecular Identification of Hepatozoon canis in Ticks from Dogs in Siirt, Turkey. test, 2022; 53(4): 541-546. doi: 10.21608/ejvs.2022.146290.1355

Molecular Identification of Hepatozoon canis in Ticks from Dogs in Siirt, Turkey

Egyptian Journal of Veterinary Sciences
Volume 53, Issue 4, December 2022, Pages 541-546    PDF (1.4 M)
Document Type: Original Article
DOI: 10.21608/ejvs.2022.146290.1355
Authors
Burçak Aslan Çelik1; Adnan Ayan2; Özgür Yaşar Çelik* 3; Ali Bilgin Yılmaz4; Özlem Orunç Kılınç5; Özge Oktay Ayan6
1Department of Parasitology, Faculty of Veterinary Medicine, Siirt University, Siirt, TURKEY
2Department of Genetics, Faculty of Veterinary Medicine, Van Yüzüncü Yıl University, Van, TURKEY
3Departmen t of Internal Medicine, Faculty of Veterinary Medicine, University of Siirt, Siirt, Turkey
4School of Health, Van Yuzuncu Yil University, Van, TURKEY
5Özalp Vocational School, Van Yüzüncü Yıl University, Van, TURKEY
6Department of Parasitology, Van Yüzüncü Yıl University, Faculty of Medicine, Van, TURKEY,
Abstract
Hepatazoon species are apicomplexan parasites belonging to the Heptazoidae family, and phylogenetically they are closely related to piroplasms and hemosporinids. This study was performed on Hepatozoon canis positive ticks collected from dogs to perform sequencing of their 18S rRNA genetic section using the PCR method. Tick collection was carried out from a total of 80 dogs in Siirt, located in the Southeastern Anatolia region of Turkey. A total of 300 collected ticks were morphologically identified to the species level and all ticks identified as R. sanguineus (s.l.). H. canis DNA was detected in 12 (%4) out of 300 in R. sanguineus ticks by PCR. The phylogenetic tree created by the comparison of amplified 18S rRNA region sequences of H. canis with MT107097.1, MH595911.1, KT215377.1, KT 215376.1, KC 584780.1, KC 584777.1, KC 584775.1, and KC 584774.1. The results obtained will provide important reference material for both veterinary physicians and dog owners in terms of managing Canine hepatozoonosis.
Keywords
Hepatozoon canis; PCR; Rhipicephalus sanguineus; Siirt; Turkey
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