M., A., Salim, D., Atwa, A. (2016). Incidence of Vibrio Species in Some Crustaceans and Molluscus. test, 2(2), 335-347. doi: 10.21608/ejceh.2016.254336
Abd-Elaaty E. M.; Dalia A. Salim; Asem M. Atwa. "Incidence of Vibrio Species in Some Crustaceans and Molluscus". test, 2, 2, 2016, 335-347. doi: 10.21608/ejceh.2016.254336
M., A., Salim, D., Atwa, A. (2016). 'Incidence of Vibrio Species in Some Crustaceans and Molluscus', test, 2(2), pp. 335-347. doi: 10.21608/ejceh.2016.254336
M., A., Salim, D., Atwa, A. Incidence of Vibrio Species in Some Crustaceans and Molluscus. test, 2016; 2(2): 335-347. doi: 10.21608/ejceh.2016.254336

Incidence of Vibrio Species in Some Crustaceans and Molluscus

Egyptian Journal of Chemistry and Environmental Health
Volume 2, Issue 2, November 2016, Pages 335-347    PDF (663.31 K)
Document Type: Scientific and Research
DOI: 10.21608/ejceh.2016.254336
Authors
Abd-Elaaty E. M.* 1; Dalia A. Salim2; Asem M. Atwa3
1Department of Food Control, Bacteriology. Animal Health Research Institute. Shebin El koom Branch, Egypt.
2Department of Food Control, Bacteriology. Animal Health Research Institute. Shebin El koom Branch, Egypt
3Department of Food Control,Bacteriology. Animal Health Research Institute. Shebin El koom Branch, Egypt
Abstract
Crustaceans and Molluscus, as Crab and Oyster are filter-feeding organisms that concentrate microorganisms, especially the human enteric viruses and pathogenic Vibrio species. This study aimed to detect and accurate identify Vibrio sp. in fresh and marine Crab and Oyster specimens especially that of public health important as cholerae, Vibrio parahaemolyticus, Vibrio mimicus and Vibrio vulnificususing using biochemical methods and PCR methods (16SrRNA and mPCR). The prevalence of Vibrio species was 5%, 50% and 65% in fresh Crab, marine Crab and Oyster samples respectively examined by traditional biochemical method appeared in examined fresh Crab samples as only one vibrio mimicus species as (5%). Vibrio species in collected marine Crab samples was V. alginolyticus (15%), V. cholera (5%), V. parahaemolyticus (10%) and V. vulnificus (20%). Vibrio species in oyster was V. alginolyticus (5%), V. cholera ( 10%), V. mimicus (5%), V. parahaemolyticus (20%) and V.vulnificus (25%). Overall, results of the present study indicated that the using of 16SrRNA and mPCR allowed rapid and accurate identification of the predominant and pathogenic Vibrio species which failed to be accurately identified by biochemical method. Furthermore, presence of Vibrio cholerae and Vibrio mimicus in examined samples considered a life threatening to human health.
Keywords
Clams; V. vulnificus; V. cholerae; V.parahaemolyticus; V. mimicus; PCR
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