PLANT REGENERATION FROM PERIANTHES ANDDIVERSE EXPLANTS FROM GLADIOLUS INFLORESCENCE | ||
| Journal of Plant Production | ||
| Article 17, Volume 27, Issue 5, May 2002, Pages 3323-3331 PDF (614.08 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jpp.2002.254716 | ||
| Author | ||
| Yasseen, Y. Mohamed | ||
| Genetic Engineering and Biotechnology Research Institute Minufiya University, Sadat City, Egypt | ||
| Abstract | ||
| A procedure for plant regeneration from perianthes and different explants of gladiolus inflorescence is described. Perianthes from different stages of development were cultured on Murashige and Skoog medium (MS) supplemented with 2 mg/l benzyladenine (BA) and 0.1 mg/l naphthaleneacetic acid (NAA). Small perianthes were cultured on MS containing 0.0, 1, 2, 3, 4, 5, 6 mg/l BA and 0.1 mg/l NAA. Inflorescence stem, inflorescence nodes, bracts and ovaries were cultured on MS supplemented with 2 mg/l BA and 0.1 mg/l NAA. Inflorescence explants and anthers were also cultured on callus-induction medium composed of MS supplemented with 1.5 mg/l dichlorophenoxyacetic acid (2,4-D), 0.5 mg/l NAA, 0.5 mg/l kinetin, 200 mg/l casein hydrolysate and 300 mg/l glutamine. Multiple shoots were regenerated from all types of inflorescence explants cultured on media containing BA and NAA. Shoot regeneration from perianthes was greatly affected by flower age and concentration of BA and NAA. Callus was produced from inflorescence explants on callus-induction medium; however, callus was not produced from anthers. Plantlets were regenerated from callus upon transfer to MS containing 0.2 mg/l BA. Produced shoots were transferred to root-induction medium composed of MS containing 0.1 mg/l indolebutyric acid (IBA). Rooted plantlets were transferred to soil and normal phenotypicplants were successfully established in soil. | ||
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