Screening of cellulolytic activity of some Rhizospheric soil fungi Isolated from different sources | ||
| Delta Journal of Science | ||
| Article 6, Volume 44, Issue 2, July 2022, Pages 88-100 PDF (543.08 K) | ||
| Document Type: Research and Reference | ||
| DOI: 10.21608/djs.2022.148953.1029 | ||
| Authors | ||
| mostafa elsheekh1; Eman Esmail Abdallah* 2; metwally abdelazeem1 | ||
| 1Botany Department, Faculty of Science, Tanta University | ||
| 2botany and microbiology department, faculty of science , Tanta university | ||
| Abstract | ||
| Cellulases are enzymes that catalyze the bioconversion of cellulose to reduce sugars and glucose. Most fungal cellulase enzymes degrade the cellulose molecule and several other polysaccharides into monosaccharides. In this study, twenty fungal species were isolated from several locations in Al-Gharbia governorate, Egypt. The soil samples are from various plant rhizospheres (rice, wheat, bean, sugar beet, and pea).These fungi were identified from seven different genera "Trichoderma, Aspergillus, Rhizopus, Cephalosporium, Fusarium, Penicillium, Mucor". All identified fungal species were tested for cellulolytic activity in the solid medium. Out of twenty fungal species, seventeen species showed cellulolytic activity and the highest activity was recorded in Trichoderma viride (67.2 U/ml) and Aspergillus sydawi (52.1 U/ml). The cellulolytic fungi were selected to estimate the carboxymethyl cellulase (CMCase), β-glucosidase (βGase) and filter paper cellulase (FPase) in the liquid medium. All tested fungi showed significant activity for the three enzymes. Trichoderma viride had the highest total cellulolytic activity of the three cellulolytic enzymes. | ||
| Keywords | ||
| Rhizospheric soil fungi; cellulolytic enzymes; Trichoderma viride | ||
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