Development of a simple and rapid method to confirm chyle in biological fluid: Short Communication | ||
| SVU-International Journal of Medical Sciences | ||
| Article 26, Volume 6, Issue 1, January 2023, Pages 250-253 PDF (370.18 K) | ||
| Document Type: Short communications | ||
| DOI: 10.21608/svuijm.2022.153279.1357 | ||
| Authors | ||
| Kranti Santosh Sorte Gawali; Avinash Namdeo Jadhao* | ||
| Department of Biochemistry, Seth GSMC & KEMH, Parel Mumbai 400008, Maharashtra , India. | ||
| Abstract | ||
| Chyle consists of lymph and emulsified fats absorbed from the intestine after digestion and absorption. Patients are often found of chyluria in endemic parts of Africa, India and southern Asia It is caused by the parasitic filarial nematode Wuchereria bancrofti (W. bancrofti). We often receive body fluids mixed with chyle eg. chyluria, chylothorax, chyloperitonium etc. in laboratory for confirmation. Well established biochemical method is there, but it demands expertise and time. To establish a simple method to confirm chyle in biological fluid .Equal volume of suspected chyle sample and ether are mixed by vigorous shaking and standing for 5 minutes, a white band appears at the junctions of two liquids. Sample taken from white band and observed under microscope. Round retractile globules can be seen. Utility of our method is that it does not demand too much of expertise, very short procedure and this method can be used in field where filariasis is endemic. | ||
| Keywords | ||
| Chyle; Ether; Rapid | ||
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