GENETIC BEHAVIOUR OF SOME EGYPTIAN COTTON VARITIES (Gossypium barbadense L.) UNDER STRESSCONDITIONS OF WADI SUDR,SOUTH SINAI | ||
| Journal of Plant Production | ||
| Article 4, Volume 27, Issue 7, July 2002, Pages 4379-4388 PDF (1.18 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jpp.2002.255601 | ||
| Author | ||
| Nahed A. K. Rashed, | ||
| Plant Genetic Resources Dept. Desert Research Center Matarlya, Cairo. | ||
| Abstract | ||
| Two successive experiments were carried out at Ras Sudr Agricultural Research Station of ORC during 2000 and 2001 growth seasons to investigate the mean performance (adaptability) of six Egyptian cotton varieties to saline conditions of Wadi Sudr. Also to evaluate the genetic variation and genetic relationships between varieties using random amplified polymorphic DNA (RAPD) markers. Long staple varieties Giza 90 then Giza 83 followed by Deodera were more adapted to saline conditions and attained the significant highest values of yield and its attributes. Meanwhile extra long staple ones Giza 77 as well as Giza 45 were less adapted and exhibited a significant lower values. Five random 10-mer primers were used to amplify DNA via polymerase chain reaction (PCR) and 55 RAPDs were generated. The five primers detected polymorphism in all six cotton varieties. Out of the total amplified bands 60 % were polymorphic. Similarity matrix was generated from the RAPD results as compared by using Nei and Li's (1979) coefficient. Giza 77 was the least similar one with other studied varieties whereas Giza 90, Giza 83 were the greatest similar varieties. Dendrogram generated. by UPGMA analysis, detected that the more adapted varieties to saline conditions. long staple varieties Giza 90, Giza 83 and Dendera grouped together; Giza 45 the extra long staple variety which and Giza 85 the long staple variety showed that significant lower yield and its attributes grouped together whereas Giza 77 extra long staple variety and lowest adapted or significant lowest yielding ability did not duster with any other variety tested. The results obtained could be used for selection the suitable parents for breeding program for improving fiber qualities and its adaptability under saline j conditions. | ||
| Keywords | ||
| Cotton (Gossypium Barbadense L.) RAPD; Genetic similarity; Genetic diversity; Yield and its attributes; Saline conditions | ||
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