SHOOT PROLIFERATION AND PLANT REGENERATION FROM GIANT TARO (Alocasia macrorrhiza L.) SCHOTT AND ENOL) | ||
| Journal of Plant Production | ||
| Article 7, Volume 27, Issue 7, July 2002, Pages 4623-4631 PDF (1.03 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jpp.2002.255605 | ||
| Author | ||
| yasseen, Mohamed Y. | ||
| Genetic Engineering and Biotechnology Research Institute Sadat City, Mlnufiya University, P.O.Box 79/22857, Egypt | ||
| Abstract | ||
| Alocasia macrorrlliza is an ornamental plant with decorative foliage. A procedure for propagation of Alocasia from shoot tip, axillary buds, stem pith, root and leaf petiole explants is described. Shoot tip and axillary buds were excised from mature plants and cultured on Murashige and Skoog medium (MS) supplemented with B.B 11M benzyladenine (BA) and 0.5 11M naphthaleneacetic acid (NAA) and incubated for eight weeks. Produced shoots were quartered. or left intact and cultured on MS alone or supplemented with 2.2, 4.4, B.8, 13.2, 17.6, 22.0, 26.4, 30.8, 35.2 11M BA with 0.5 11M NAA. Shoot regeneration was obtained from stem pith explants cultured on MS alone or supplemented with 0.9. 2.2, 4.4. 8.8.13.3,17.6,22.0 JIM BA with 0.5 11M NAA. Callus was produced from shoot tip, leaf, root and stem pith explants cultured on MS supplemented with different concentration from 2,4-0, pidoram or NAA. Shoot regeneration from callus occurred upon transfer into MS containing 2.2 JIM SA. Produced shoots were rooted, acdimatized, and normal plants were successfully established in soil. | ||
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