NON CONVENTIONAL METHODS FOR EXTRACTING PROTEIN FROM COWPEA LEAVES: 1- OPTIMUM CONDITIONS FOR PROTEIN ISOLATION | ||
| Journal of Food and Dairy Sciences | ||
| Article 1, Volume 27, Issue 7, July 2002, Pages 4763-4769 PDF (656.77 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jfds.2002.255916 | ||
| Authors | ||
| Fatma EI-Zahraa A. Abd EI Aal,; Salwa O. Rofael | ||
| Department of Hort. Crops Processing, Food Tec. Research Inst., ARC, Egypt. | ||
| Abstract | ||
| Chemical composition and some antinutrational factors of cowpea leaves (Vigna sinensis, Savi) , as well as setting up the optimum conditions for maximum protein extraction were studied. The electrophoretic properties of protein extraction were also investigated . Our results led to the following conclusions: - Cowpea leaves had a good source of leaf protein because it's content was 30.40% as crude protein. The highest extractable protein yeild at SO' C, after 90 minuets and pH 9 was found to be the optimum value for maximum recovery using distilled water in a ratio 1 :25 (w/w) ( cowpea leaves pwoder : solvent) , Most of protein fractions of cowpea leaves were focused in about 6 bands in the pH range of 3.5 - 4.7. Protein extract was preclpated according to it's iso electric point which found at pH 4. | ||
| Keywords | ||
| Cowpea leaves; leaf protein; protein isolate; protein electrophoresis | ||
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