USAGE OF ELECTROMAGNETIC FIELD AS A TOOL FOR DENATURATION OF GENOMIC DNA FROM Saccharomyces cerevisiae. | ||
| Journal of Agricultural Chemistry and Biotechnology | ||
| Article 1, Volume 27, Issue 7, July 2002, Pages 4923-4933 PDF (1.32 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jacb.2002.255926 | ||
| Author | ||
| S. Sharaf EI-Deen, | ||
| Microbial Genetics Department, National Research Centre, Cairo, Egypt. | ||
| Abstract | ||
| This study reports the effect of the energy of magnetic fields for usage as a denaturing agent of genetic material (DNA) from Saccharomyces.cerevisiae. Two different flux densities (0.1 and 0.5 T units) of magnetic field were applied to two different haploid yeast strains (Ura 1 and Gm3) for both 5 minutes and 15 minutes. The absorption spectra of DNA exposed to the magnetic field was analyzed at 230nm,260 nm, and 280 nm, at two different temperatures (35°C and 72"C). Absorbance spectra thus generated was compared to corresponding spectra obtained from S.cerevisiae DNA ,not exposed to magnetic field. At 720C absorbance was much higher in DNA samples exposed to magnetic field after their purification. Much higher impact of magnetic field was observed in the strain having more mutants. The magnetic field increased the energy of hydrogen bonds separated or break its and hard DNA double helix (denaturation). This effect depends on the genetic background. | ||
| Keywords | ||
| Electromagnetic field; Yeast; DNA denaturation | ||
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