MITODEPRESSIVE EFFECT AND DNA IHIBITION INDUCED BY CAMEL URINE IN A. CEPA ROOT MERISTEM CELLS. | ||
| Journal of Plant Production | ||
| Article 9, Volume 27, Issue 8, August 2002, Pages 5249-5268 PDF (2.18 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jpp.2002.256232 | ||
| Author | ||
| Sanaa A. Riad, | ||
| Dept. of Genetics, Fac.of Agric., Alex. Univ., Alexandria, Egypt. | ||
| Abstract | ||
| Camel urine is used by Beduines in the treatment of many diseases. since it contains chemical compounds affect the physiology of human body. Therefore, the effects of camel urine on the process of mitosis and chromosomal behaviour in normal cells (the root meristem cells of A. cepe) were studied in direct and recovery experiments. Also. an attempt was made to find out a relationship between the mitotic activity and the DNA content. The results clearly indicated genotoxic activity of camel urine however no clastogenic effect was observed. The observed mitodepressive effect depends on the concentration and exposure time of direct treatment and it may be due to the presence of camel urine cytotoxic constituents such as purine bases. hypoxanthine and creatinine. The non-clastogenic nature probably was attributed to the antioxidant and the antimutagenic compounds (uric acid and creatinine) present in camel urine. The mitostalic action may be due to an accumulation of clumping stickimetaphase cells. Thus. chromosomes lose the ability to undergo anaphase and telophase stages and are completely arrested at metaphase in cells treated with high doses for long exposure time. Beside stickiness, other physiological aberrations were observed which probably attributed to abnormal spindle formation. On the other hand. the mitostatic feature may be due to the reduction in the number of cells entering mitosis and the depression of DNA synthesis at S phase. During recovery periods, the process of mitosis and DNA concentration were recuperate in meristem cells treated with 10% of camel urine. On the contrary. the attempt to revive the meristem cells was not effective for the sublethal dose (20%). It revealed complete genotoxic effect and continuous decrease of DNA content at all recovery times. Further studies are in progress to explain the genotoxic activety of camel urine and its active compounds on the cell cycle phases and gene expression. | ||
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