EVALUATION OF ADVANCED GENERATIONS OF EARLY FLOWERING AND HEAVY BRANCHING/HIGH YIELDING MUTANTS OF EGYPTIAN LUPINE II-CHEMICAL FEATURES OF MUTANTS | ||
| Journal of Plant Production | ||
| Article 11, Volume 27, Issue 10, October 2002, Pages 6745-6763 PDF (4.82 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jpp.2002.256530 | ||
| Authors | ||
| M. A. Nassar,; H. R. Hassan | ||
| Department of Agricultural Botany, Faculty of Agriculture, Cairo University, Giza, Egypt | ||
| Abstract | ||
| Four mutants (two early flowering and two heavy branching/high yield) obtained from Egyptian lupine cv.Giza 2 after treating seeds with either ethyl methane sulphonate (EMS) or sodium azide (SA), Hassan (1991 and 1998) in a mutation breeding programme conducted at the Agricultural Experiments and Researches Station, Faculty of Agriculture, University of Cairo, Giza, Egypt since 1987. In M7- generation, these mutants were evaluated for alkaloid and fatty acid composition of the oil. GLC analysis proved the presence of six alkaloids in lupine seeds under investigation. Lupanine represents the major component (56.32-63.34%) in all investigated seeds followed by 13&-hydroxylupanine (12.40-17.32%) then by multiflorine (8.64-13.74). Whereas, albine (3.38-9.02), angustifoline (4.09-6.75%) and 11,12-Seco-12,13-didehydromultiflorine (2.59-5.93%) represent the minor components. It is noted that the increase in total alkaloid content, which was observed in seeds of the heavy branching/high yield (G2 EMS HS/HY6), early flowering (G2 EMS EF1) ana early flowering (G2 SA EF2) mutants, was reflected in increased concentration of individual components. Likewise, the reduction in total alkaloid content, which was observed in the heavy branching/high yield (G2 SA HS/HY3), was reflected in reduced concentration of individual components. On the other hand, GLC analysis proved the presence of seven fatty acids beside two unknowns in lupine seeds under investigation. The higher relative concentration was for fatty acid C18:1 (39.6-47.9%) in all investigated seeds, followed by C18:2 (21.4-24.8%), while C16:0 and C18:3 reached (16.6-18.2%) and (8.3-12.4%); respectively. The other fatty acids, C18:0, C20:0, C22:0, unknown1 and unknown 2 were present in lesser amounts in all investigated seeds ranged from traces to 2.2%. It is noted that the increase in total fatty acids content, which was observed in seeds of the heavy branching/high yield and early flowering mutants, reflected in increased concentration of individual components. | ||
| Keywords | ||
| Evaluation; early flowering; heavy branching/high yielding; mutants; Egyptian lupine; chemical features | ||
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