Development HPLC technique for determining Oxymetazoline and Isoxspurine in pharmaceutical formulations | ||
| Egyptian Journal of Chemistry | ||
| Article 67, Volume 65, Issue 11, November 2022, Pages 779-784 PDF (194.31 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejchem.2022.151275.6551 | ||
| Authors | ||
| Zaid Alnedawi1; Ali M Hassan1; Hind Hadi2; Ahmed Shabana1 | ||
| 1Department of Chemistry, Faculty of Science, Al-Azhar University, Cairo, Egypt | ||
| 2Department of Chemistry, College of Science, Baghdad University, Baghdad, Iraq | ||
| Abstract | ||
| A fast high-performance liquid chromatography method was established to estimate Oxymetazoline hydrochloride and Isoxsuprine hydrochloride in pharmaceutical preparations HPLC. It consists of an Azorbax-C8 (250mm ×4.6 mm, 5µm ) column with a mobile phase composed of methanol:acetonitrile: buffer (0.01 M) potassium dihydrogen phosphate with triethylamine (pH 5.5) (50: 10:40, V/V/V ) eluted at 1.5 ml/min flow rate. The detection was done using a photodiode array (PDA) detector. The proposed method was validated in linearity, precision, and accuracy. The retention times of IsoxsuprineHCl and OxymetazolineHCl are 3.6 and 6.2 ±0.4 min, respectively. The linear range and detection limits were 1-250 µg/ml and 0.5 µg/ml, respectively, for both drugs. The proposed method was successfully applied to determine ISX and OXY in pharmaceutical preparations. | ||
| Keywords | ||
| Oxymetazoline Hydrochloride; HPLC; Isoxsuprine Hydrochloride | ||
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