Design of a new rapid and efficient kit for extracting DNA from blood sample | ||
| Egyptian Journal of Chemistry | ||
| Volume 66, Issue 5, May 2023, Pages 107-110 PDF (424.74 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejchem.2022.151209.6546 | ||
| Authors | ||
| Shahad A. Jarallah1; Dheaa Sh. Zgeer1; Falah S. Al-Fartusie2 | ||
| 1Al-Nahrain University, College of Science, Department of Chemistry, Baghdad, Iraq | ||
| 2Mustansiriya University, College of Science, Department of Chemistry, Baghdad, Iraq | ||
| Abstract | ||
| Extraction of DNA with a pure and high and product is a main goal for researchers in the field of molecular biology or forensic science. Over recent years, isolation of pure, intact, and highly concentrated genomic DNA has been a prerequisite for successful and reliable genetic analysis. The classical DNA extraction method involves the use of organic and inorganic reagents such as chloroform andphenol, which have a toxic effect on humans. Many of the current modern technologies rely on physical extraction, which has contributed greatly to the development of methods for dealing with DNA, such as extraction based on magnetic beads or extraction based on cellulose paper.Based on the wide range of options available in automated DNA extraction protocols, it would be ideal to select those that offer the best cost-effectiveness and time-efficiency performance. The aim of this research was to design a new set to extract DNA from whole blood samples in an easy and highly efficient manner in terms of the amount of DNA extracted and the time required to perform the extraction. In addition, it is economically suitable in terms of cost, based on the physical method of DNA isolation using silica-coated magnetic beads. On this basis, the main conclusion of this work, it can be drawn that a new kit of DNA extraction has been prepared with good properties. The new kit is easy to use, less expensive, safe and offers a good yield | ||
| Keywords | ||
| DNA extraction; DNA concentration; gelelectrophorysis; Nanodrop | ||
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