Studies on Incidence and Prevention of Nosocomial Infection of Urinary Tract Endoscopies by Different Antimicrobial Agents | ||
| Egyptian Journal of Microbiology | ||
| Article 8, Volume 47, Issue 1, December 2012, Pages 127-140 PDF (609.38 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejm.2012.256 | ||
| Abstract | ||
| THIRTY FIVE bacterial isolates were collected from urine and blood samples of 20 patients, before and after endoscopy examination, in new Surgical Hospitals , Zagazig University Hospital, Zagazig, Egypt. Antibiotic susceptibility profile of purified bacteria revealed four multi-resistant strains identified as Staphylococcus aureus Zag11, Pseudomonas aeruginosa Zag60, Escherichia coli Zag126 and Staphylococcus epidermidis Zag128. The four selected bacteria were subjected to some disinfectants (glutaraldehyde, hydrogen peroxide, P3-oxonia and Orthophthaladehyde) at different concentrations and different exposure times. It was observed that 10 min were enough to inhibit the growth of tested pathogenic bacteria in case of (8% H2O2 & 0.55% orthophthaladehyde) while completely inhibition was recorded after 15min in the case of (2.2% glutaraldehyde, 70% ethanol, and 0.45% P3-oxonia). Sterile urinary tract endoscopy was artificially contaminated with mixture (1:1:1:1:1:1) of six clinical pathogenic bacterial strains comparing with the four tested bacterial strains. Upon exposing the contaminated endoscope to different chemical disinfectants; 8% hydrogen peroxide and 0.55% Orthophthalaldehyde inhibited after 30 min exposure while 2.2% Glutaraldehyde, 0.45% P3oxonia, and 70% Ethanol needed 60 min for complete bacterial inhibition. Upon exposure of artificially contaminated endoscope to different physical agents (U.V, γ- rays and dry hot air), Gamma rays showed maximum inhibitory action. | ||
| Keywords | ||
| Nosocomial infection; Urinary tract endoscopies; antimicrobial agent | ||
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