The Role of N-Acetyl Cysteine in Ameliorating Doxorubicin Induced Cardiotoxicity in Rats | ||
| Zagazig Journal of Forensic Medicine and Toxicology | ||
| Article 17, Volume 20, Issue 2, July 2022, Pages 319-338 PDF (1.35 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/zjfm.2021.71789.1075 | ||
| Authors | ||
| Emadeldin Mahmoud* 1; Randa Hussien2; Safaa George3; Abeer Refaiy4; Rania Ahmed Radwan5 | ||
| 1Aswan university faculty of medicine. forensic medicine and clinical toxicology department | ||
| 2Forensic medicine and Clinical Toxicology department, Faculty of medicine, Assiut University. Assiut city Egypt | ||
| 3forensic medicine and clinical toxicology, faculty of medicine, assiut university. Assiut city. | ||
| 4Pathology department, Faculty of medicine, Assiut University Assiut city | ||
| 5Forensic Medicine and Clinical Toxicology, Faculty of medicine, Sohag University, Sohag, Egypy | ||
| Abstract | ||
| Doxorubicin (DOX) is an anthracycline antibiotic and a quinone-containing chemotherapeutic drug used for various types of solid and hematological cancers. However, it causes many toxic side effects, including cardiac, renal, hematological, and testicular. The current study investigated the ameliorative effect of n-acetylcysteine against DOX-induced cardiotoxicity in albino rats. Sixty rats were divided into six groups. Group (A): rats received food pellets and tap water ad libitum. Group (B): rats received NAC (400 mg/kg/p.o.) Every other day for 28 days. Group (C): rats received DOX (5 mg/kg, i.p.) for four weeks on days 1, 7, 14 and 21. Group (D): rats received NAC (400 mg/kg/p.o.) every other day one week earlier and then along with DOX (5 mg/kg/ i.p.) for the next four weeks on days 1, 7, 14 and 21). Group (E): rats received NAC (400 mg/kg/ p.o.) every other day started at the first day of the experiment till the end of the experiment and DOX (5 mg/kg/ i.p.) for four weeks on day 1, 7, 14 and 21. Group (F): rats received NAC (800 mg/kg, p.o.) every other day started at the first day of the experiment till the end of the experiment and DOX (5 mg/kg, i.p.) for four weeks on day 1, 7, 14 and 21. Results showed a significant increase in levels of LDH, CK-MB, CTn-I, and (MDA). Whilst, levels of (GSH) and (GSH-Px) were significantly decreased. Moreover, there were histopathological abnormalities in the cardiac tissue of DOX-treated rats. The study demonstrated that NAC has a cardioprotective effect on the damage induced by DOX, through inhibition of inflammation and oxidative stress. This effect was more pronounced in groups (D, E&F), as it produced a significant increase in GSH and GSH-Px levels, a significant decrease in MDA, LDH, CK-MB, and CTn-I. | ||
| Keywords | ||
| Doxorubicin; N-Acetyl cysteine; cardiotoxicity; Oxidative stress | ||
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