Dimethylsulfoxide vs. 1, 2 Propandiol as Cryoprotective Additives during Vitrification in Cryopreservation of Human Embryos | ||
| The Egyptian Journal of Fertility and Sterility | ||
| Article 2, Volume 17, Issue 1 - Serial Number 11106352, January 2013, Pages 7-10 PDF (1.33 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/egyfs.2013.257397 | ||
| Author | ||
| Mohamed M Shaaban* 1, 2 | ||
| 11 Department of Chemistry, Faculty of Science, Ain Shams University, Cairo, Egypt | ||
| 22 Obstetrics and Gynecology Department; Faculty of Medicine; Suez Canal University | ||
| Abstract | ||
| Objective: Assessment of the survival rate of human embryos after vitrification/thaw process using two different freezing (vitrification) media with two different solvents; Dimethylsulfoxide (DMSO) based medium and 1,2Propandiol (PROH) based medium. These two solvents act as cryoprotective additives (CPA) that provide a protective effect for human embryos vitrification. Subject & Methods: Day2 or day3 divided human embryos were exposed to DMSO or PROH based media before plunging into Liquid Nitrogen (LN2) storage. Later on, during replacement cycles, the embryos were thawed and their survival rate was evaluated. Results: Two hundreds and twelve embryos in vitrification process were into two groups. Group A (114 embryos) were treated with sequential vitrification medium based on DMSO and Group B (98 embryos) were treated with PROH based medium. Thawed embryos were transferred to physiological media designed for cleavage stage embryos and kept in the CO2 incubator for 2 to 8 hours. After vitrification/thawing procedure, the survival rates of group A (DMSO) and group B (PROH) were 85 survived embryos of 114 (74.6%) and 52 survived embryos of 98 (52%) respectively (P value<0.05). Conclusion: DMSO based medium used for vitrification/thaw process of day2 or day 3 divided embryos demonstrated a significant higher embryo survival rate than that of PROH based vitrification medium. | ||
| Keywords | ||
| Keywords: Embryo cryopreservation; Dimethylsulfoxide; DMSO; 1, 2 Propandiol; PROH; Vitrification | ||
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