SOME ASPECTS OF IN VITRO MICROPROPAGATION OF DATE PALM (Phoenix dactylifera L.) | ||
| Journal of Plant Production | ||
| Article 10, Volume 26, Issue 9, September 2001, Pages 5449-5466 PDF (808.59 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jpp.2001.257777 | ||
| Authors | ||
| A. M. Abd El-Hamid,1; M. H. A. Abou-Bakr2; I. A. Ibrahim2; M. A. Abd El-Baky3 | ||
| 1Dept. of Agric. Botany, Fac. Of Agric., Cairo University, Giza, Egypt. | ||
| 2Genetic Engineering and Biotechnology Research Institute, Menofyia Univ., Egypt. | ||
| 3Ministry of Agriculture and Soil Reclamation, Giza, Egypt | ||
| Abstract | ||
| The effect of callus source (shoot tip, axillary bud and leaf primordium), medium (growth regulators) and number of subcultures on both somatic embryogenesis and shoot formation were studied. Moreover, the anatomical features of roots and leaves of regenerated plantlets were also studied in comparison with those of seedlings. Results revealed that shoot tip callus on a medium containing 2mg/L IBA gave the best somatic embryogenesis. Hormone free medium and that containing 5 mg/L 2, ip did not cause somaclonal variation, while on other media it ranged between 14.42 and 32.15%. On transferring onto free hormone medium, 20% of the abnormal embryos developed normal plantlets. The highest rate of shoot multiplication was obtained with shoot tip callus on medium containing 3mg/L 2,ip. Comparative anatomy of in vitro and ex vitro leaves and roots, indicated that the leaves of the in vitro regenerated plantlets had well developed tissues. On the contrary, their roots were characterized by poorly developed cortex and vascular bundles, which may be the main causes of the observed problems with plantlet acclimatization. Abbreviations. 2,4-D: 2,4 dichlorophenoxyacetic acid; NAA: naphthaleneacetic acid; IBA: indole-3- butyric acid; 2, ip: (2-dimethylallayl – amino)- purin; GA3: gibberellic acid. | ||
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