INFLUENCE OF CULTIVATION CONDITIONS ON THE PRODUCTION OF Aspergillus niger M 2 CELLULASES | ||
| Journal of Agricultural Chemistry and Biotechnology | ||
| Article 1, Volume 26, Issue 9, September 2001, Pages 5749-5760 PDF (676.33 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jacb.2001.257987 | ||
| Author | ||
| Nadia A. A. Ali, | ||
| Microbiol. Dept., Soil, Water and Environ. Res. Institute, Agric. Res. Center, Giza, Egypt. | ||
| Abstract | ||
| Screening of sixteen fungal strains for their cellulases potentiality revealed that Aspergillus niger M2 was the most active strain for b–glucosidase, CMCase and FPase production. 1% corn stalk induced greatly A. niger cellulases. Barley flour, corn flour and corn stalk were found as the best raw materials, which induced the biosynthesis of all three enzymes examined. Sugar cane molasse (1%) as carbon source had given highest amount of three cellulases which stimulated its production. The presence of corn steep liquor at 0.056% as nitrogen content in the production medium has very necessary for highest biosynthesis of A. niger cellulases. 0.310% and 0.155% as phosphorus content of KH2PO4 have very necessary for b–glucosidase and CMCase & FPase, respectively. Maximum enzyme productivity was obtained at pH 6.5 and 7.5% of inoculum level to the production medium solution. 35°C and 200 rpm/min was found as the suitable incubation temperature and agitation rate for cellulases biosynthesis. 96 hours was found as the best incubation period for enzyme formation. | ||
| Keywords | ||
| Aspergillus niger; optimizing; cellulase; -glucosidase; carboxy methyl cellulase (CMCase); filter paper cellulase (FPase)–production; bioconversion; plant raw materials | ||
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