TRIAL FOR ISOLATION AND CHARACTERIZATION OF PDXVIRUS FROM TURKEYS | ||
| Egyptian Journal of Agricultural Research | ||
| Article 26, Volume 82, Issue 2, July 2004, Pages 869-880 PDF (2.23 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejar.2004.258366 | ||
| Authors | ||
| OLFAT E. NAKHLA; AHMED M. DAOUD | ||
| Veterinary Serum and Vaccine Research Institute, Agricultural Research Center, Ministry of Agriculture, Dokki, Giza, Egypt | ||
| Abstract | ||
| Turkeys showing lesions of pox infection were subjected to postmortem examination and samples were collected for isolation of the causative agent. An isolate was successfully recovered from the cutaneous pox lesions in embryonated chicken eggs .The isolate was tested in comparison with virulent fowl poxvirus for pathogenicity in susceptible birds (Turkeys, fowls and ducks). It was found that it produced pock lesions on the chorioallantoic membrane of ECE and specific takes at the site of inoculation by the wing-web method in fowls and ducks , and generalized proliferative nodules on the unfeathered parts of inoculated turkeys . On the other hand , inoculation of fowl poxvirus in the same birds ( turkeys , fowls and ducks ) induced generalized pox lesions which appeared on turkeys and fowls, while the ducks did not produce any reaction. These results confirmed the identity of the isolate as turkey poxvirus. Further confirmation that the isolate belongs to avian poxviruses was achieved by histopathological examination of samples collected from organs of experimentally infected turkeys and detection of the virus antigen in the organs of experimentally infected turkeys by the indirect fluorescent antibody technique. | ||
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