Identification of Histopathological Changes Induced by Amitraz in Rats | ||
| Journal of Applied Veterinary Sciences | ||
| Article 13, Volume 7, Issue 4, October 2022, Pages 88-93 PDF (767.58 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/javs.2022.154375.1169 | ||
| Authors | ||
| Zainab Taha Mohammed* 1; Rizgar Khalid Nabi2; Yaareb Jaafar Mousa3; Mahmood Basher Mahmood1 | ||
| 1Department of Internal Medicine, Surgery and Pharmacology, College of Veterinary Medicine, University of Dohuk, Duhok, Iraq. | ||
| 2Department of Pathology and Microbiology, Collage of Veterinary Medicine, University of Duhok, Duhok, Iraq. | ||
| 3Department of Physiology, Biochemistry and Pharmacology, College of Veterinary Medicine, University of Mosul, Mosul, Iraq. | ||
| Abstract | ||
| Amitraz is used worldwide as a pesticide, it produces toxic effects when misused. The peresent study examined the histopathological changes of amitraz in the hepatocyte and kidney tissues of the laboratory albino rats. Thirty-six albino rats were used and they were randomly divided into six groups. Amitraz was given through oral gavage at different doses as follows: The control group (group one) received 5ml/kg of normal saline, group two received 100mg/kg, group three had 250mg/kg, group four received 500mg/kg , organs extraction from the rats (livers and kidneys) were performed after three hours from giving amitraz administration in groups one, two, three, and four. Group five received 500mg/kg and had organ collection after 5 hours of administration, group six received the same as last dose and had organ collection after 24 hours of administration. The results revealed that amitraz produced severe histopathological effects on the liver and kidney tissues compared to the control group. The changes were obvious in rats treated with high dose groups. Severe and significant histopathological changes were evident in the hepatocyte and kidney tissues in the group six of the treated rats compare to the group 5 and 4 of rats. While the lowest given dose group showed normal tissues, indicating that amitraz requires a larger quantity to produce the histopathological action. The study concluded that amitraz has hepatotoxic and nephrotoxic effects when overdose is used. | ||
| Keywords | ||
| Amitraz; Histopathology; Hepatotoxicity; Nephrotoxicity; Rats | ||
| References | ||
|
AGUWA, U. S., EZE, C. E., OBINWA, B. N., OKEKE, S. N., ONWUELINGO, S. F., OKONKWO, D. I., and UMEZULIKE, A., 2020. Comparing the effect of methods of rat euthanasia on the brain of Wistar rats: Cervical dislocation, chloroform inhalation, diethyl ether inhalation and formalin inhalation. J Adv Med Res, 32, 8-16.
https://Doi: 10.9734/jammr/2020/v32i1730636
AL-HAMMDANI, YA., and AL-BAGGOU, B.K., 2014. Study of acute toxicosis and biochemical changes induced by Amitraz in chicks. Iraqi Journal of Veterinary Sciences 28(2). https://Doi:10.33899/ijvs.2014.116929.
AL-THANI, R.K., AL-THANI, A.S., ELBETIEHA, A., and DARMANI, H., 2003. Assessment of reproductive and fertility effects of amitraz pesticide in male mice. Toxicology Letters 138(3), 253-260. https://Doi: 10.1016/s0378-4274(02)00418-6.
DHOORIA, S., BEHERA, D., and AGARWAL, R., 2015. Amitraz: a mimicker of organophosphate poisoning. Case Reports, 2015, bcr2015210296. https://Doi.org/10.1136/bcr-2015-210296
EPSTEIN, S. E., POPPENGA, R., and STUMP, S., 2021. Amitraz toxicosis in 3 dogs after being in a rice field. Journal of Veterinary Emergency and Critical Care, 31(4), 516-520. https://Doi.org/10.1111/vec.13072.
EUROPEAN FOOD SAFETY AUTHORITY (EFSA) 2016. Setting of maximum residue levels for Amitraz, coumaphos, flumequine, oxytetracycline, permethrin and streptomycin in certain products of animal origin. EFSA Journal, 14(8), e04570. https://Doi.org /10.2903/j.efsa.2016.4570.
FILAZI, A., GÜVENÇ, T., KUM, C., and SEKKIN, S., 2004. Pathological findings in acute amitraz intoxication in mice. Turkish Journal of Veterinary & Animal Sciences, 28(5), 873-878. https://journals.tubitak.gov.tr/veterinary/vol28/iss5/14
FILAZI, A., and YURDAKOK-DIKMEN, B., 2018. AMITRAZ. IN VETERINARY TOXICOLOGY (PP. 525-531). Academic Press. https://Doi.org/10.1016/B978-0-12-811410-0.00041-6
GUPTA, R. C., 2017. Toxicity of pesticides. In Lu's Basic Toxicology (pp. 453-485). CRC Press. https://Doi.org/10.4324/9781315391700
HERATH, H. M. M. T. B., PAHALAGAMAGE, S. P., YOGENDRANATHAN, N., WIJAYABANDARA, M. D. M. S., and KULATUNGA, A., 2017. Amitraz poisoning: A case report of an unusual pesticide poisoning in Sri Lanka and literature review. BMC pharmacology and toxicology, 18(1), 1-6. https://Doi.org/10.1186/s40360-016-0114-5.
IKEN, I., ABDESSADEK, M., EL ATTARI, A., and ACHOUR, S., 2020. Poisoning by Amitraz, uncommon pesticide revealed by high-performance liquid chromatography: About two cases. Toxicologie Analytique et Clinique, 32(3), 200-204. https://Doi.org/10.1016/j.toxac.2019.12.001.
KIZIL, O., BALIKCI, E., DABAK, M., and OZDEMIR, H., 2008. Amitraz intoxication in two cattle. Revue Méd. Vét, 159(3), 166-168. https://www.researchgate.net/publication/288613669.
KRIEGER, R. 2010. Hayes' handbook of pesticide toxicology (Vol. 1). Academic Press.
LUNA, L. G. 1968. Manual of histologic staining methods of the Armed Forces Institute of Pathology.
MAHMOOD, M. B., and MOHAMMED, Z. T., 2019. determination of LD50 and acute toxicity effects on some biochemical parameters induced by Amitraz in rats. Bas.J.Vet.Res.Vol.18, No.2.
MARAFON, C. M., DELFIM, C. I. G., VALADAO, C. A. A., MENOTTI, R., and ANDRADE, S. F., 2010. Analysis of Amitraz in cats by gas chromatography. Journal of veterinary pharmacology and therapeutics, 33(4), 411 414. https://Doi: 10.1111/j.1365-2885.2009.01144.x.
MULLIN, C. A., FRAZIER, M., FRAZIER, J. L., ASHCRAFT, S., SIMONDS, R., VANENGELSDORP, D., and PETTIS, J. S., 2010. High levels of miticides and agrochemicals in North American apiaries: implications for honey bee health. PloS one, 5(3), e9754. https://Doi.org/10.1371/journal.pone.0009754.
MUSTAFA, K. A., and AL-BAGGOU, B. K., 2020. Toxicological and neurobehavioral effects of chlorpyrifos and deltamethrin insecticides in mice. Iraqi Journal of Veterinary Sciences, 34(1), 189-196. https:/Doi: 10.33899/ijvs.2019.125738.1144
NANJUNDAPPA, S., NAIR, S. N., UDAYAN, D., KANAPADINCHAREVEETIL, S., JACOB, M., RAVINDRAN, R., and JULIET, S., 2021. Disposition Kinetics of Amitraz in Lactating Does. Molecules, 26(16), 4769. https://Doi.org/10.3390/molecules26164769.
OMOJA, V. U., ASUZU, I. U., and ANIKA, S. M., 2016. Assessment of the hepatic and renal effects of sub-chronic administration of sub-lethal doses of amitraz/xylene in albino Wistar rats. Comparative Clinical Pathology,25(1), 203-209. https://Doi.org/10.1007/s00580-015-2170-4.
SHILPAKAR, O., KARKI, B., and RAJBHANDARI, B., 2020. Amitraz poisoning-tale of an unusual pesticide poisoning: a case report.JNMA: Journal of the Nepal Medical Association,58(225), 338. https://Doi.org/10.31729/jnma.4919.
SHIMSHONI, J. A., SPERLING, R., MASSARWA, M., CHEN, Y., BOMMURAJ, V., BORISOVER, M., and BAREL, S., 2019. Pesticide distribution and depletion kinetic determination in honey and beeswax: Model for pesticide occurrence and distribution in beehive products. PLoS One,14(2), e0212631. https://Doi.org/10.1371/journal.pone.0212631.
TARALLO, V. D., LIA, R. P., SASANELLI, M., CAFARCHIA, C., and OTRANTO, D., 2009. Efficacy of Amitraz plus Metaflumizone for the treatment of canine demodicosis associated with Malassezia pachydermatis. Parasites &Vectors,2(1),1-5. https://Doi.org/10.1186/1756-3305-2-13.
VUCINIC, S., JOVANOVIC, D., VUCINIC, Z., JOKSOVIC, D., SEGRT, Z., ZLATKOVIC, M., and JOVANOVIC, M., 2007. A near-fatal case of acute poisoning by amitraz/xylene showing atrial fibrillation. Forensic Toxicology,25(1), 41-44. https://Doi.org/10.1007/s11419-006-0019-x.
WILSON, W., and MURTY, S., 2018. Amitraz poisoning: The (Un) common poisoning. Journal of Emergencies, Trauma, and Shock,11(2), 140. https://Doi: 10.4103/jets.jets_33_17
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