THE USE OF BANANA PEEL FOR CELLULOLYTIC ENZYMES PRODUCTION BY A LOCAL STRAIN OF Aspergillus niger | ||
| Journal of Agricultural Chemistry and Biotechnology | ||
| Article 7, Volume 25, Issue 10, October 2000, Pages 6503-6512 PDF (646.62 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jacb.2000.259830 | ||
| Authors | ||
| F. I. A. Hauka,; A. E. I. Selim; Samia M. M. Bayoumy | ||
| Agric. Microbiol. Dept., Fac. of Agric., Mansoura Univ, Mansoura, Egypt. | ||
| Abstract | ||
| Banana peel was used as a carbon source to produce cellulases and cellobiase in shake flask cultures by A. niger. Peak cellulases and cellobiase activities were obtained on the 6th day of fermentation. The temperature and pH influenced the yield of enzymes. The maximum cellulase activities were recorded at 30°C and pH 5.0. The maximum rate of enzyme activities was obtained when A. niger was grown on 1.5% banana peel. Deprivation of ammonium sulphate, peptone or nitrogen source as whole inhibited cellulolytic enzyme formation. The maximum enzyme activities were recorded at 40°C except cellobiase, which had maximum activity at 30°C. The optimum pH of CMCase, FPase and cellobiase was 5.5, 6.5 and 5.5, respectively. The maximum CMCase and cellobiase activities were detected after 1 h, whereas the maximum FPase was detected after 16 h. More than 50% of CMCase, FP-activity and cellobiase retained at 60, 70 and 60°C, respectively. | ||
| Keywords | ||
| Banana peel; cellulolytic enzymes; Aspergillus niger; carboxymethyl cellulase; filter paper-ase; cellobiase | ||
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