Mukriani, M., Ahmad, A., Asmi, N., Karim, H., Massi, M., Prihantono, P. (2022). Production and characterization of L-Asparaginase enzyme from symbiont bacteria of red algae Eucheuma spinosum. test, 65(11), 475-481. doi: 10.21608/ejchem.2022.81705.4045
Mukriani Mukriani; Ahyar Ahmad; Nur Asmi; Harningsih Karim; Muhammad Nasrum Massi; Prihantono Prihantono. "Production and characterization of L-Asparaginase enzyme from symbiont bacteria of red algae Eucheuma spinosum". test, 65, 11, 2022, 475-481. doi: 10.21608/ejchem.2022.81705.4045
Mukriani, M., Ahmad, A., Asmi, N., Karim, H., Massi, M., Prihantono, P. (2022). 'Production and characterization of L-Asparaginase enzyme from symbiont bacteria of red algae Eucheuma spinosum', test, 65(11), pp. 475-481. doi: 10.21608/ejchem.2022.81705.4045
Mukriani, M., Ahmad, A., Asmi, N., Karim, H., Massi, M., Prihantono, P. Production and characterization of L-Asparaginase enzyme from symbiont bacteria of red algae Eucheuma spinosum. test, 2022; 65(11): 475-481. doi: 10.21608/ejchem.2022.81705.4045

Production and characterization of L-Asparaginase enzyme from symbiont bacteria of red algae Eucheuma spinosum

Egyptian Journal of Chemistry
Article 43, Volume 65, Issue 11, November 2022, Pages 475-481    PDF (602.24 K)
Document Type: Original Article
DOI: 10.21608/ejchem.2022.81705.4045
Authors
Mukriani Mukriani1; Ahyar Ahmad* 2; Nur Asmi2; Harningsih Karim3; Muhammad Nasrum Massi4; Prihantono Prihantono5
1Graduate School of Medical Science, Medicine Faculty, Hasanuddin University, Perintis Kemerdekaan Street Km. 10 Tamalanrea, Makassar 90245, Indonesia
2Chemistry Department, Mathematics and Natural Sciences, Hasanuddin University, Makassar, Indonesia
3Pharmacy College YAMASI, Makassar 90222, Indonesia
4Department of Microbiology, Faculty of Medicine, Hasanuddin University, Makassar 90245, Indonesia
5Department of Oncology, Medicine Faculty, Hasanuddin University, Perintis Kemerdekaan Street Km. 10 Tamalanrea, Makassar 90245, Indonesia
Abstract
Red algae are one of the marine creatures which can produce a variety of both primary and secondary compounds. It is widely known that the existence of exploitation will cause species extinction if there is no cultivation. Due to this problem, the use of bacterial symbionts of algae is one of the solutions. This study aimed to obtain bacteria isolates that can produce the L – Asparaginase (L-ASNase) enzyme. The research design was explorative using experimental laboratory methods by using solid substrate fermentation techniques. The sample used was red algae Eucheuma spinosum located on the island of Lae-Lae of Spermonde island of South Sulawesi. Analysis of the data displayed in the form of tables, graphs/images. Based on the results, two species of bacteria-derived L-Asparaginae enzyme. Protein content with the highest of the bacteria Enterobacter genus obtained from fractions 20-40% was equal to 1.780 mg/mL. The highest enzyme activity was in fractions of 20-40% with 32.011 IU/mL. The optimum temperature and pH of each was at 37 °C and pH 7.
Keywords
Red algae; Bacteria; L–Asparaginase; Enzyme; Eucheuma spinosum
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