Micropropagation of Blackberry (Rubus fruticosus.) cv. Karaka Black | ||
| Egyptian Journal of Horticulture | ||
| Volume 49, Issue 2, August 2022, Pages 187-198 PDF (1.61 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejoh.2022.151882.1205 | ||
| Authors | ||
| Mina Samaan Farag Samaan1; Mohamed abd elhamed Nasser* 2 | ||
| 1Faculty of Agriculture, Ain Shams University | ||
| 2Department of Horticulture, Faculty of Agriculture, Ain Shams University, Cairo, Egypt. | ||
| Abstract | ||
| This work aimed to establish an in vitro protocol for Blackberry “Karaka Black” micropropagation. Stem node explants were experimented on three types of medium salts (WPM, MS and B5) in establishment stage. Also, three types of cytokinins were tested for multiplication i.e. BA (0.0, 0.4, 0.8 and 1.2 ppm), TDZ (0.0, 0.1, 0.5 and 1.0 ppm) and Kin (0.0, 1.0, 5.0 and 10.0 ppm). Two soil mixtures were used in acclimatization stage. Finally, the effect of passage time and number of subcultures on multiplication rate were investigated. The results indicated that WPM followed by MS medium gave the highest shoot length and leaf number. BA at 0.4 ppm, Kin at 5.0 ppm and TDZ at 0.1 ppm achieved the most preferable values for multiplication stage but the kinetin one gave the healthiest plantlets. The most microshoots got well-formed roots in multiplication phase. Consequently, rooting stage was not required. The multiplication rate slightly decreased after the 3rd subculture and three months of culture date proved to be the most suitable passage time among subcultures. Peatmoss and sand mixture gave the highest plantlet quality during acclimatization. | ||
| Keywords | ||
| Blackberry; Rubus sp; Micropropagation; In vitro Propagation; Acclimatization | ||
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